Co-Authors:
Seabra, S.G., Computational Biology and Population Genomics Group, Centro de Biologia Ambiental, Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal
Brás, P.G., Computational Biology and Population Genomics Group, Centro de Biologia Ambiental, Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal
Zina, V., Centro de Estudos Florestais, Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Lisboa, Portugal
Borges da Silva, E., Centro de Estudos Florestais, Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Lisboa, Portugal
Rebelo, M.T., Centro de Estudos do Ambiente e do Mar, Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal
Figueiredo, E., Centro de Engenharia dos Biossistemas, Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Lisboa, Portugal
Mendel, Z., Department of Entomology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Paulo, O.S., Computational Biology and Population Genomics Group, Centro de Biologia Ambiental, Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal
Franco, J.C., Centro de Estudos Florestais, Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Lisboa, Portugal
Abstract:
The occurrence of polyandry in Planococcus citri, presumed by earlier observations of mating behavior, was confirmed using microsatellite genotyping of pools of over 400 eggs resulting from controlled crosses of one female with two males. The genetic contribution of both mated males was confirmed in 13 out of 43 crosses. In three crosses it was possible to determine that only the first male fertilized the eggs, which may be due to sperm competition or unviable sperm supply. The microsatellite analysis also allowed the confirmation of aspects of the chromosomal inheritance detected previously in cytogenetic studies in Planococcus citri, namely that only one of the alleles is transmitted by the male, indicating that the males are functionally haploid, supporting the observation of Paternal Genome Elimination (PGE) in these insects. © 2013 Seabra et al.