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Characterization of newly synthesized RNA in insects by chromatography on columns of methylated albumin kieselgur and the study of its base composition
Year:
1971
Source of publication :
Insect Biochemistry
Authors :
Shaaya, Eli
;
.
Volume :
1
Co-Authors:
Shaaya, E., Departments of Physiological Chemistry and Pharmacology, University of Marburg, Lahn, Germany
Schnieders, B., Departments of Physiological Chemistry and Pharmacology, University of Marburg, Lahn, Germany
Kunz, W., Departments of Physiological Chemistry and Pharmacology, University of Marburg, Lahn, Germany
Sekeris, C.E., Departments of Physiological Chemistry and Pharmacology, University of Marburg, Lahn, Germany
Facilitators :
From page:
113
To page:
121
(
Total pages:
9
)
Abstract:
RNA, obtained by extraction from Calliphora erythrocephala larvae pulse-labelled with 32P-orthophosphate for different time periods, was analysed by MAK-column chromatography. After a 15-minute pulse most of the labelled RNA was recovered from the column by elution with 1 M ammonia. The remaining labelled RNA was found in the 30 S fraction. With longer orthophosphate pulses, labelled RNA appears in the 4-5 S region and also in the DNA-RNA hybrid fraction. Base analysis of the RNA recovered from the 1 M ammonia peak has a high AU/GC content of 57 per cent, which remains constant up to 6 hours of labelling. The 30 S peak shows a similar AU content in the short pulses as the 1 M ammonia fraction, and decreases to 48 per cent after 6 hours of labelling. The 4-5 S fraction has a high G content in comparison with the other RNA fractions. The labelling of RNA from the integument of prepupae is higher than from 5-day-old larvae. The reverse is seen in respect to fat-body RNA. © 1971.
Note:
Related Files :
DNA-like RNA
ecdysone
epidermis
fat-body
MAK-column
rapidly labelled RNA
RNA base analysis
RNA sucrose gradient
Show More
Related Content
More details
DOI :
10.1016/0020-1790(71)90027-8
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
26562
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:23
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Scientific Publication
Characterization of newly synthesized RNA in insects by chromatography on columns of methylated albumin kieselgur and the study of its base composition
1
Shaaya, E., Departments of Physiological Chemistry and Pharmacology, University of Marburg, Lahn, Germany
Schnieders, B., Departments of Physiological Chemistry and Pharmacology, University of Marburg, Lahn, Germany
Kunz, W., Departments of Physiological Chemistry and Pharmacology, University of Marburg, Lahn, Germany
Sekeris, C.E., Departments of Physiological Chemistry and Pharmacology, University of Marburg, Lahn, Germany
Characterization of newly synthesized RNA in insects by chromatography on columns of methylated albumin kieselgur and the study of its base composition
RNA, obtained by extraction from Calliphora erythrocephala larvae pulse-labelled with 32P-orthophosphate for different time periods, was analysed by MAK-column chromatography. After a 15-minute pulse most of the labelled RNA was recovered from the column by elution with 1 M ammonia. The remaining labelled RNA was found in the 30 S fraction. With longer orthophosphate pulses, labelled RNA appears in the 4-5 S region and also in the DNA-RNA hybrid fraction. Base analysis of the RNA recovered from the 1 M ammonia peak has a high AU/GC content of 57 per cent, which remains constant up to 6 hours of labelling. The 30 S peak shows a similar AU content in the short pulses as the 1 M ammonia fraction, and decreases to 48 per cent after 6 hours of labelling. The 4-5 S fraction has a high G content in comparison with the other RNA fractions. The labelling of RNA from the integument of prepupae is higher than from 5-day-old larvae. The reverse is seen in respect to fat-body RNA. © 1971.
Scientific Publication
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