אסיף מאגר המחקר החקלאי

Characterization of newly synthesized RNA in insects by chromatography on columns of methylated albumin kieselgur and the study of its base composition

Year:

1971

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Volume :

1

Co-Authors:

Shaaya, E., Departments of Physiological Chemistry and Pharmacology, University of Marburg, Lahn, Germany
Schnieders, B., Departments of Physiological Chemistry and Pharmacology, University of Marburg, Lahn, Germany
Kunz, W., Departments of Physiological Chemistry and Pharmacology, University of Marburg, Lahn, Germany
Sekeris, C.E., Departments of Physiological Chemistry and Pharmacology, University of Marburg, Lahn, Germany

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Abstract:

RNA, obtained by extraction from Calliphora erythrocephala larvae pulse-labelled with 32P-orthophosphate for different time periods, was analysed by MAK-column chromatography. After a 15-minute pulse most of the labelled RNA was recovered from the column by elution with 1 M ammonia. The remaining labelled RNA was found in the 30 S fraction. With longer orthophosphate pulses, labelled RNA appears in the 4-5 S region and also in the DNA-RNA hybrid fraction. Base analysis of the RNA recovered from the 1 M ammonia peak has a high AU/GC content of 57 per cent, which remains constant up to 6 hours of labelling. The 30 S peak shows a similar AU content in the short pulses as the 1 M ammonia fraction, and decreases to 48 per cent after 6 hours of labelling. The 4-5 S fraction has a high G content in comparison with the other RNA fractions. The labelling of RNA from the integument of prepupae is higher than from 5-day-old larvae. The reverse is seen in respect to fat-body RNA. © 1971.

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