אסיף מאגר המחקר החקלאי

Advanced backcross QTL analysis of a Lycopersicon esculentum x Lycopersicon parviflorum cross

Year:

2000

Source of publication :

Authors :

Volume :

100

Co-Authors:

Fulton, T.M., Dept. of Plant Breeding and Biometry, 252 Emerson Hall, Cornell University, Ithaca, NY 14853, United States
Grandillo, S., CNR-IMOF, Res. Inst. Veg. Ornamental Plant B., Via Universita' 133, 80055, Portici (Naples), Italy
Beck-Bunn, T., Seminis Vegetable Seeds, 37537 State Highway 16, Woodland, CA 95659, United States
Fridman, E.
Frampton, A., Seminis Vegetable Seeds, 37537 State Highway 16, Woodland, CA 95659, United States
Lopez, J., Nestle R and D Center S.A., Apartado 435, E-06080, Badajoz, Spain
Petiard, V., Francerco, 101 Avenue Gustave Eiffel, 37390 Notre Dame, Tours, France
Uhlig, J., Seminis Vegetable Seeds, 37537 State Highway 16, Woodland, CA 95659, United States
Zamir, D., Hebrew University, Faculty of Agriculture, Department of Field Crops, Rehovot 76-100, Israel
Tanksley, S.D., Dept. of Plant Breeding and Biometry, 252 Emerson Hall, Cornell University, Ithaca, NY 14853, United States

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Abstract:

Lycopersicon parviflorum is a sexually compatible, wild tomato species which has been largely unutilized in tomato breeding. The Advanced Backcross QTL (AB-QTL) strategy was used to explore this genome for QTLs affecting traits of agronomic importance in an interspecific cross between a tomato elite processing inbred, Lycopersicon esculentum E6203, and the wild species L. parviflorum (LA2133). A total of 170 BC2 plants were genotyped by means of 133 genetic markers (131 RFLPs; one PCR-based marker, 1-2, and one morphological marker, u, uniform ripening). Approximately 170 BC3 families were grown in replicated field trials, in California, Spain and Israel, and were scored for 30 horticultural traits. Significant putative QTLs were identified for all traits, for a total of 199 QTLs, ranging from 1 to 19 QTLs detected for each trait. For 19 (70%) traits (excluding traits for which effects of either direction are not necessarily favourable or unfavourable) at least one QTL was identified for which the L. parviflorum allele was associated with an agronomically favourable effect, despite the overall inferior phenotype of the wild species.

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