Co-Authors:
Yemets, A., Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine
Stelmakh, O., Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine
Blume, Y.B., Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine
Abstract:
To clarify the mechanism of isopropyl-N-phenyl carbamate (IPC) action on higher plant cells the sensitivity of microtubules (cortical network and mitotic arrays) and microtubule organizing centers to IPC treatment (30 μM) in IPC-resistant and sensitive Nicotiana sylvestris lines was studied. It was clearly demonstrated that IPC does not depolymerize plant MTs but causes the MTOC damage in cells, which results in MTOC fragmentation, splitting of the spindle poles and in abnormal division spindle formation. It was also found that IPC-resistance of mutant N. sylvestris line correlates not with tubulin resistance to IPC action but possibly with resistance of one of the proteins involved in MTOC composition. © 2008 International Federation for Cell Biology.