Co-Authors:
Peerenboom, E., Max-Planck-Inst. Zuchtungsforschung, Abt. Genetische Grundlagen P., Carl von-Linne-Weg 10, 50829 Köln, Germany
Cartwright, E.J., Crop and Dis. Management Department, IACR-Rothamsted, Harpenden, Herts ALS 2JQ, United Kingdom
Foulds, I., PBI Cambridge, Maris Lane, Trumpington, Cambridge CB2 2LQ, United Kingdom
Adams, M.J., Crop and Dis. Management Department, IACR-Rothamsted, Harpenden, Herts ALS 2JQ, United Kingdom
Stratford, R., PBI Cambridge, Maris Lane, Trumpington, Cambridge CB2 2LQ, United Kingdom
Rosner, A., Virology Department, Volcani Centre, Bet-Dagan 50250, Israel
Steinbiss, H.-H., Max-Planck-Inst. Zuchtungsforschung, Abt. Genetische Grundlagen P., Carl von-Linne-Weg 10, 50829 Köln, Germany
Antoniw, J.F., Crop and Dis. Management Department, IACR-Rothamsted, Harpenden, Herts ALS 2JQ, United Kingdom
Abstract:
The complete RNA1 sequences of two isolates (fungus transmissible and non-fungus transmissible) of barley mild mosaic virus (BaMMV) were obtained. The two isolates' RNA1 sequences had very high sequence identity (99,3%), and of the 15 amino acid differences (out of 2258), between the putative polyproteins, 11 were conservative and unlikely to affect the structure or function of the protein. The remaining amino acid differences were thought unlikely to affect fungus transmission because they occur in the CI- and NIb- coding regions. This strongly suggests that the P73 protein of RNA2 (which has a 364-aa deletion in the non-fungus-transmissible isolate) is involved in fungus transmission of BaMMV.