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Isolation of a citrus promoter specific for reproductive organs and its functional analysis in isolated juice sacs and tomato
Year:
2011
Source of publication :
Plant Cell Reports
Authors :
Bardosh, Gabriel
;
.
Fridman, Ifat
;
.
Or, Etti
;
.
Sadka, Avi
;
.
Shlizerman, Lyudmila A.
;
.
Sorkina, Alina
;
.
Zur, Naftali
;
.
Volume :
30
Co-Authors:
Sorkina, A., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Bardosh, G., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Liu, Y.-Z., Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Ministry of Education, Wuhan 430070, China
Fridman, I., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Schlizerman, L., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Zur, N., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Or, E., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Goldschmidt, E.E., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Blumwald, E., Department of Plant Sciences, University of California, One Shields Ave, Davis, CA 95616, United States
Sadka, A., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
1627
To page:
1640
(
Total pages:
14
)
Abstract:
While searching for genes expressed in acid lemon but not in acidless lime pulp, we isolated clone Cl111 which showed the following expression phenotypes: (1) while it was expressed in the ovaries in both varieties, its mRNA was detected only in the pulp of the acid fruit, (2) no or very low expression of the gene was detected in vegetative organs. These expression patterns suggested that Cl111 is an ovary- and pulp-specific gene. The ability of ~2-kb fragments upstream of the transcription start site of the lemon and lime genes to confer reporter-gene activity was investigated by transient expression in isolated juice vesicles of both varieties. Whereas Cl111 promoter from lemon showed faint activity in lemon and lime juice vesicles, no activity was evident with the lime promoter. The activities of the 2-kb fragments and their delimited fragments were further investigated in tomato. The results indicated that the promoters were active in a manner similar to that in acid lemon and acidless lime: the lemon promoter generated activity in the fruit endocarp, analogous to citrus fruit pulp. The delimitation analyses identified an expression-conferring region which, in the lemon promoter, contained a sequence homologous to a fruit-specific element of the melon cucumisin gene. Another region, which reduced promoter activity, contained an I-Box-like sequence, identified as a fruit-specific negative element. Taken together, Cl111 promoter was confirmed to be pulp- and flower-specific. Differences in the expression of Cl111 between the two varieties could be attributable to changes in the gene promoter region. © 2011 Springer-Verlag.
Note:
Related Files :
Base Sequence
Citrus limon
Genetics
genetic transformation
metabolism
molecular genetics
Show More
Related Content
More details
DOI :
10.1007/s00299-011-1073-3
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
26701
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:24
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Scientific Publication
Isolation of a citrus promoter specific for reproductive organs and its functional analysis in isolated juice sacs and tomato
30
Sorkina, A., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Bardosh, G., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Liu, Y.-Z., Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Ministry of Education, Wuhan 430070, China
Fridman, I., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Schlizerman, L., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Zur, N., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Or, E., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Goldschmidt, E.E., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Blumwald, E., Department of Plant Sciences, University of California, One Shields Ave, Davis, CA 95616, United States
Sadka, A., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Isolation of a citrus promoter specific for reproductive organs and its functional analysis in isolated juice sacs and tomato
While searching for genes expressed in acid lemon but not in acidless lime pulp, we isolated clone Cl111 which showed the following expression phenotypes: (1) while it was expressed in the ovaries in both varieties, its mRNA was detected only in the pulp of the acid fruit, (2) no or very low expression of the gene was detected in vegetative organs. These expression patterns suggested that Cl111 is an ovary- and pulp-specific gene. The ability of ~2-kb fragments upstream of the transcription start site of the lemon and lime genes to confer reporter-gene activity was investigated by transient expression in isolated juice vesicles of both varieties. Whereas Cl111 promoter from lemon showed faint activity in lemon and lime juice vesicles, no activity was evident with the lime promoter. The activities of the 2-kb fragments and their delimited fragments were further investigated in tomato. The results indicated that the promoters were active in a manner similar to that in acid lemon and acidless lime: the lemon promoter generated activity in the fruit endocarp, analogous to citrus fruit pulp. The delimitation analyses identified an expression-conferring region which, in the lemon promoter, contained a sequence homologous to a fruit-specific element of the melon cucumisin gene. Another region, which reduced promoter activity, contained an I-Box-like sequence, identified as a fruit-specific negative element. Taken together, Cl111 promoter was confirmed to be pulp- and flower-specific. Differences in the expression of Cl111 between the two varieties could be attributable to changes in the gene promoter region. © 2011 Springer-Verlag.
Scientific Publication
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