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ACTH induces TIMP-1 expression and inhibits collagenase in adrenal cortex cells
Year:
2004
Authors :
Reichenstein, Moshe
;
.
Volume :
215
Co-Authors:
Reichenstein, M., Department of Molecular Biology, College of Judea and Samaria, Ariel 44837, Israel
Reich, R., Department of Pharmacology, Hebrew University, Jerusalem, Israel
Lehoux, J.-G., Department of Biochemistry, Faculty of Medicine, Universite de Sherbrooke, Que., Canada
Hanukoglu, I., Department of Molecular Biology, College of Judea and Samaria, Ariel 44837, Israel
Facilitators :
From page:
109
To page:
114
(
Total pages:
6
)
Abstract:
To identify genes that are induced by corticotropin (ACTH) in adrenal cortex cells, we carried out a differential hybridization screening of adrenal cortex cDNA libraries. Some of the clones we identified represented tissue inhibitor of metalloproteinase 1 (TIMP-1) mRNA. We examined ACTH dependence of the expression of TIMP-1 in vitro in cultured bovine adrenocortical cells, and in ACTH-treated rats. Northern blot analysis of total RNA from cells showed that the level of TIMP-1 mRNA increases sharply within 3h after ACTH stimulation. Since TIMP-1 inhibits some cell matrix metalloproteinases (MMPs) of the collagenase type, we examined the effect of ACTH on collagenase activity in bovine adrenocortical cells. Exposure of confluent cultures to ACTH for 24h showed dose-dependent inhibition of collagenase activity. Northern blot analysis of total RNA from rat adrenal zona fasciculata-reticularis and zona glomerulosa showed that in both of these zones TIMP-1 expression was induced within 12h after ACTH injection. Long-term (9 days) treatment with ACTH increased TIMP-1 mRNA levels nearly sixfold in zona fasciculata-reticularis. Overall, our results show that ACTH causes induction of TIMP-1 and suppression of collagenase activity, and suggest that ACTH may modulate the activities of MMPs and hence cell matrix remodeling. © 2003 Elsevier Ireland Ltd. All rights reserved.
Note:
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More details
DOI :
10.1016/j.mce.2003.11.011
Article number:
Affiliations:
Database:
Scopus
Publication Type:
Conference paper
;
.
Language:
English
Editors' remarks:
ID:
26739
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:25
Scientific Publication
ACTH induces TIMP-1 expression and inhibits collagenase in adrenal cortex cells
215
Reichenstein, M., Department of Molecular Biology, College of Judea and Samaria, Ariel 44837, Israel
Reich, R., Department of Pharmacology, Hebrew University, Jerusalem, Israel
Lehoux, J.-G., Department of Biochemistry, Faculty of Medicine, Universite de Sherbrooke, Que., Canada
Hanukoglu, I., Department of Molecular Biology, College of Judea and Samaria, Ariel 44837, Israel
ACTH induces TIMP-1 expression and inhibits collagenase in adrenal cortex cells
To identify genes that are induced by corticotropin (ACTH) in adrenal cortex cells, we carried out a differential hybridization screening of adrenal cortex cDNA libraries. Some of the clones we identified represented tissue inhibitor of metalloproteinase 1 (TIMP-1) mRNA. We examined ACTH dependence of the expression of TIMP-1 in vitro in cultured bovine adrenocortical cells, and in ACTH-treated rats. Northern blot analysis of total RNA from cells showed that the level of TIMP-1 mRNA increases sharply within 3h after ACTH stimulation. Since TIMP-1 inhibits some cell matrix metalloproteinases (MMPs) of the collagenase type, we examined the effect of ACTH on collagenase activity in bovine adrenocortical cells. Exposure of confluent cultures to ACTH for 24h showed dose-dependent inhibition of collagenase activity. Northern blot analysis of total RNA from rat adrenal zona fasciculata-reticularis and zona glomerulosa showed that in both of these zones TIMP-1 expression was induced within 12h after ACTH injection. Long-term (9 days) treatment with ACTH increased TIMP-1 mRNA levels nearly sixfold in zona fasciculata-reticularis. Overall, our results show that ACTH causes induction of TIMP-1 and suppression of collagenase activity, and suggest that ACTH may modulate the activities of MMPs and hence cell matrix remodeling. © 2003 Elsevier Ireland Ltd. All rights reserved.
Scientific Publication
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