אסיף מאגר המחקר החקלאי

Next-generation sequencing-based QTL mapping for unravelling causative genes associated with melon fruit quality traits

Year:

2017

Source of publication :

Authors :

Volume :

1151

Co-Authors:

Portnoy, V., Department of Vegetable Research, Newe Yáar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay, Israel
Gonda, I., Department of Vegetable Research, Newe Yáar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay, Israel
Galpaz, N., Department of Vegetable Research, Newe Yáar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay, Israel, Northern Agriculture RandD, PO Box 831, Kiryat Shmona, Israel
Tzuri, G., Department of Vegetable Research, Newe Yáar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay, Israel
Lev, S., Department of Vegetable Research, Newe Yáar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay, Israel
Kenigswald, M., Department of Vegetable Research, Newe Yáar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay, Israel, Department of Postharvest and Food Sciences, ARO, Volcani Center, Agricultural Research Organization, PO Box 6, Bet Dagan, Israel
Fei, Z., Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, United States
Barad, O., NRGENE, 3 Golda Meir St. Park HaMada, Ness Ziona, Israel
Harel-Beja, R., Department of Vegetable Research, Newe Yáar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay, Israel
Doron-Faigenboim, A., Department of Vegetable Research, Volcani Center, Agricultural Research Organization, PO Box 6, Bet Dagan, Israel
Bar, E., Department of Vegetable Research, Newe Yáar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay, Israel
Sáar, U., Department of Vegetable Research, Newe Yáar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay, Israel
Xu, Y., Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, United States
Lombardi, N., Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, United States
Mao, L., Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, United States
Jiao, C., Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, United States
Kol, G., NRGENE, 3 Golda Meir St. Park HaMada, Ness Ziona, Israel
Gur, A., Department of Vegetable Research, Newe Yáar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay, Israel
Fallik, E., Department of Postharvest and Food Sciences, ARO, Volcani Center, Agricultural Research Organization, PO Box 6, Bet Dagan, Israel
Tadmor, Y., Department of Vegetable Research, Newe Yáar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay, Israel
Burger, Y., Department of Vegetable Research, Newe Yáar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay, Israel
Schaffer, A.A., Department of Vegetable Research, Volcani Center, Agricultural Research Organization, PO Box 6, Bet Dagan, Israel
Giovannoni, J., Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, United States
Lewinsohn, E., Department of Vegetable Research, Newe Yáar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay, Israel
Katzir, N., Department of Vegetable Research, Newe Yáar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay, Israel

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Abstract:

High resolution QTL mapping of genetic factors affecting fruit quality traits in melon (Cucumis melo) was achieved in two projects. Next-generation sequencing (NGS), coupled with unique genetic materials, was used to attain high resolution mapping, in both studies. The first project was based on the RNA-seq of mature fruits of a Recombinant Inbred Lines (RIL) population derived from a cross between PI 414723 and 'Dulce'. The second project was based on genotyping-by-sequencing (GBS) analyses of F3 plants derived from a cross between 'Noy Amid' and 'Dulce'. In each project, QTL analysis was conducted using approximately 60,000 SNP markers that were detected by either GBS or RNA-seq. In the first project, phenotyping and metabolic profiling included fruit quality traits associated with taste, aroma and color while the second project focused on flesh and rind color, ethylene and the formation of the fruit abscission zone. Evidence of the high resolution of the analysis was provided in cases where QTLs for selected fruit traits were mapped to an interval of 1- 20 genes that included the known underlying causative genes. These cases included genes associated with the accumulation of specific aroma volatiles and fruit fleshcolor.

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