Co-Authors:
Shaaya, E., The Volcani Center, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50-250, Israel
Shirk, P.D., Insect Attractants, Behavior, and Basic Biology Research Laboratory, ARS-USDA, Gainesville, FL 32604, U.S.A.
Zimowska, G., Insect Attractants, Behavior, and Basic Biology Research Laboratory, ARS-USDA, Gainesville, FL 32604, U.S.A., Department of Invertebrate Physiology, University of Warsaw, Warszawa, Poland
Plotkin, S., The Volcani Center, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50-250, Israel
Young, N.J., Department of Biochemistry, University of Liverpool, P.O. Box 147, Liverpool, L69 3BX England, United Kingdom
Rees, H.H., Department of Biochemistry, University of Liverpool, P.O. Box 147, Liverpool, L69 3BX England, United Kingdom
Silhacek, D.L., Insect Attractants, Behavior, and Basic Biology Research Laboratory, ARS-USDA, Gainesville, FL 32604, U.S.A.
Abstract:
Photo-synchronized pupae were used to determine the titer of ecdysteroids during the pupal and pharate adult stages of the Indianmeal moth, Plodia interpunctella (Hübner). For female pupae that were kept in a 16 h light:8 h dark (long-day) photocycle, there was one major peak of ecdysteroids. The ecdysteroids reached a maximum of 2000-2500 pg/mg wet wt between 28-36 h after pupation. The ecdysteroids declined to < 500 pg/mg wet wt by 68 h after pupation. For pupae that were kept in continuous darkness, the ecdysteroid peak became broader with a maximum at 24 h (3130 pg/mg wet wt). The composition of ecdysteroids at various times during pharate adult development was determined using gas chromatograph-mass spectrometry with selected ion monitoring [GC-MS (SIM)]. Ecdysone was the major component (93%) in samples taken 28 h after pupalation, at the maximum of the pupal peak. Thus, high levels of ecdysone are correlated with the beginning of adult ovarian development. As pharate development progressed, the proportion of 20-hydroxyecdysone increased so that by 100 h after pupation 20-hydroxyecdysone was the only ecdysteroid detected. Previously, the initiation of vitellogenesis was determined to begin between 96 and 100 h after pupation. In conjuction with previous work, these findings demonstrate that the ecdysteroid titers decline before vitellogenesis is initiated and egg maturation can be completed. © 1993.
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