אסיף מאגר המחקר החקלאי

Sulfide induction of synthesis of a periplasmic protein in the cyanobacterium Oscillatoria limnetica.

Year:

1989

Source of publication :

Authors :

Volume :

171

Co-Authors:

Arieli, B., Division of Microbial and Molecular Ecology, Hebrew University of Jerusalem, Israel.
Binder, B., Division of Microbial and Molecular Ecology, Hebrew University of Jerusalem, Israel.
Shahak, Y., Division of Microbial and Molecular Ecology, Hebrew University of Jerusalem, Israel.
Padan, E., Division of Microbial and Molecular Ecology, Hebrew University of Jerusalem, Israel.

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Abstract:

Two proteins which may play a role in the induction of anoxygenic photosynthesis in Oscillatoria limnetica have been demonstrated by comparing the pattern of labeling during pulses of [35S]methionine of cells incubated under inducing conditions [anaerobic conditions plus 3-(3,4-dichlorophenyl)-1,1-dimethylurea, light, and sulfide) with that of cells incubated under noninducing conditions (without sulfide). The major inducible protein has an apparent molecular mass of 11.5 kilodaltons and is associated with a less strongly labeled 12.5-kilodalton protein. The synthesis of both proteins commences within the first 30 min of induction and continues throughout the 2-h induction period. Since these proteins are not synthesized in the presence of dithionite without sulfide, low redox potential alone is insufficient as an inducer of these proteins. Lysozyme treatment and/or osmotic shock of intact cells results in the release of the sulfide-induced proteins. Our data thus indicate that these proteins are located in the periplasmic space of the cells.

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