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Moskovitz, Y., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Goszczynski, D.E., Virology Unit, Agricultural Research Council, Plant Protection Research Institute, Pretoria, South Africa
Bir, L., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Fingstein, A., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Czosnek, H., Robert H. Smith Institute of Plant Science and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot, Israel
Mawassi, M., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel, Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Grapevine virus B (GVB) has been found associated with corky bark-diseased vines. Although the sequence of a 7.6-kb cDNA clone from a GVB isolate from Italy has been described, striking differences in sequences between GVB isolates prompted us to construct an additional full-length GVB clone from the isolate 94/971 and to determine its complete sequence. The cDNA of GVB 94/971 shared a nucleotide sequence identity of only 77% with the GVB isolate from Italy. The cDNA of GVB 94/971 was infectious on Nicotiana plants as demonstrated by symptoms and by means of Northern blot, Western blot and electron microscopic analyses. © 2007 Springer-Verlag.
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Sequencing and assembly of a full-length infectious clone of grapevine virus B and its infectivity on herbaceous plants
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Moskovitz, Y., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Goszczynski, D.E., Virology Unit, Agricultural Research Council, Plant Protection Research Institute, Pretoria, South Africa
Bir, L., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Fingstein, A., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Czosnek, H., Robert H. Smith Institute of Plant Science and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot, Israel
Mawassi, M., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel, Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Sequencing and assembly of a full-length infectious clone of grapevine virus B and its infectivity on herbaceous plants
Grapevine virus B (GVB) has been found associated with corky bark-diseased vines. Although the sequence of a 7.6-kb cDNA clone from a GVB isolate from Italy has been described, striking differences in sequences between GVB isolates prompted us to construct an additional full-length GVB clone from the isolate 94/971 and to determine its complete sequence. The cDNA of GVB 94/971 shared a nucleotide sequence identity of only 77% with the GVB isolate from Italy. The cDNA of GVB 94/971 was infectious on Nicotiana plants as demonstrated by symptoms and by means of Northern blot, Western blot and electron microscopic analyses. © 2007 Springer-Verlag.
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