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Sequencing and assembly of a full-length infectious clone of grapevine virus B and its infectivity on herbaceous plants
Year:
2008
Source of publication :
Archives of Virology
Authors :
Bir, Lena
;
.
Fenigstein, Anni
;
.
Mawassi, Munir
;
.
Moskovitz, Yoni
;
.
Volume :
153
Co-Authors:
Moskovitz, Y., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Goszczynski, D.E., Virology Unit, Agricultural Research Council, Plant Protection Research Institute, Pretoria, South Africa
Bir, L., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Fingstein, A., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Czosnek, H., Robert H. Smith Institute of Plant Science and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot, Israel
Mawassi, M., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel, Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
323
To page:
328
(
Total pages:
6
)
Abstract:
Grapevine virus B (GVB) has been found associated with corky bark-diseased vines. Although the sequence of a 7.6-kb cDNA clone from a GVB isolate from Italy has been described, striking differences in sequences between GVB isolates prompted us to construct an additional full-length GVB clone from the isolate 94/971 and to determine its complete sequence. The cDNA of GVB 94/971 shared a nucleotide sequence identity of only 77% with the GVB isolate from Italy. The cDNA of GVB 94/971 was infectious on Nicotiana plants as demonstrated by symptoms and by means of Northern blot, Western blot and electron microscopic analyses. © 2007 Springer-Verlag.
Note:
Related Files :
Genetics
Grapevine virus B
molecular genetics
Molecular Sequence Data
Nicotiana
Virology
virus RNA
Show More
Related Content
More details
DOI :
10.1007/s00705-007-1075-1
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
26966
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:27
You may also be interested in
Scientific Publication
Sequencing and assembly of a full-length infectious clone of grapevine virus B and its infectivity on herbaceous plants
153
Moskovitz, Y., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Goszczynski, D.E., Virology Unit, Agricultural Research Council, Plant Protection Research Institute, Pretoria, South Africa
Bir, L., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Fingstein, A., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Czosnek, H., Robert H. Smith Institute of Plant Science and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot, Israel
Mawassi, M., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel, Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Sequencing and assembly of a full-length infectious clone of grapevine virus B and its infectivity on herbaceous plants
Grapevine virus B (GVB) has been found associated with corky bark-diseased vines. Although the sequence of a 7.6-kb cDNA clone from a GVB isolate from Italy has been described, striking differences in sequences between GVB isolates prompted us to construct an additional full-length GVB clone from the isolate 94/971 and to determine its complete sequence. The cDNA of GVB 94/971 shared a nucleotide sequence identity of only 77% with the GVB isolate from Italy. The cDNA of GVB 94/971 was infectious on Nicotiana plants as demonstrated by symptoms and by means of Northern blot, Western blot and electron microscopic analyses. © 2007 Springer-Verlag.
Scientific Publication
You may also be interested in