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Protoplasma
Umiel, N., Department of Field and Vegetable Crops, The Hebrew University, Faculty of Agriculture, Rehovot, Israel
Goldner, R., Department of Field and Vegetable Crops, The Hebrew University, Faculty of Agriculture, Rehovot, Israel
Diploid callus cultures from four genetic lines of tobacco (Nicotiana tabacum) were grown in the presence of 0-2 mg/ml streptomycin sulfate. These genetic lines are closely related with regard to nuclear genes, but differ in their respective cytoplasmic genomes. In calli from all these four lines, the formation of green color was inhibited at 0.5 mg/ml streptomycin. Small differences were observed between the four lines in their respective growth on medium containing streptomycin. Streptomycin resistant mutants were isolated from cultures at high drug concentrations, using as criteria for isolation either the ability of the callus to grow green, or a better rate of growth as indicated by the size of white callus. © 1976 Springer-Verlag.
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Effects of streptomycin on diploid tobacco callus cultures and the isolation of resistant mutants
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Umiel, N., Department of Field and Vegetable Crops, The Hebrew University, Faculty of Agriculture, Rehovot, Israel
Goldner, R., Department of Field and Vegetable Crops, The Hebrew University, Faculty of Agriculture, Rehovot, Israel
Effects of streptomycin on diploid tobacco callus cultures and the isolation of resistant mutants
Diploid callus cultures from four genetic lines of tobacco (Nicotiana tabacum) were grown in the presence of 0-2 mg/ml streptomycin sulfate. These genetic lines are closely related with regard to nuclear genes, but differ in their respective cytoplasmic genomes. In calli from all these four lines, the formation of green color was inhibited at 0.5 mg/ml streptomycin. Small differences were observed between the four lines in their respective growth on medium containing streptomycin. Streptomycin resistant mutants were isolated from cultures at high drug concentrations, using as criteria for isolation either the ability of the callus to grow green, or a better rate of growth as indicated by the size of white callus. © 1976 Springer-Verlag.
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