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Tiron as a substrate for mushroom tyrosinase
Year:
1987
Source of publication :
Phytochemistry
Authors :
Kahn, Varda
;
.
Volume :
26
Co-Authors:
Kahn, V., Dept. of Food Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel
W. Miller, R., Dept. of Food Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel, Plant Research Center, Agriculture Canada, Ontario, Canada KIA OC6
Facilitators :
From page:
2459
To page:
2466
(
Total pages:
8
)
Abstract:
Tiron (4,5-dihydroxy-1,3-benzene disulphonic acid) can serve as a substrate for mushroom tyrosinase with a Km value of 34 mM. The product(s) formed is yellow (λmax 435 nm) and is stable with time. An intermediate product, having significant absorbance at 3685 nm (probably tiron-o-quinone), was detected and the kinetics of its conversion to the final yellow product(s) was studied. Hydrogen peroxide (at 0.03-1.3 mM) accelerated the conversion of the intermediate compound to the yellow product(s). Tiron-semiquinone was detected by EPR spectroscopy during the initial phase of Tiron oxidation by mushroom tyrosinase. Maximum EPR signal intensity due to Tiron-semiquinone and the time required to reach maximum intensity were dependent on the amount of mushroom tyrosinase but not on the presence or absence of hydrogen peroxide (3.3 mM). In view of separate studies showing that the yellow product(s) is mainly a low M, polymerized Tiron-quinone, suggestions as to possible pathways by which such a product(s) is formed are discussed. © 1987.
Note:
Related Files :
intermediate (λmax 365 nm)
Mushroom
Tiron
Tyrosinase
yellow product (λmax 435 nm).
Show More
Related Content
More details
DOI :
10.1016/S0031-9422(00)83853-4
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
27130
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:28
Scientific Publication
Tiron as a substrate for mushroom tyrosinase
26
Kahn, V., Dept. of Food Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel
W. Miller, R., Dept. of Food Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel, Plant Research Center, Agriculture Canada, Ontario, Canada KIA OC6
Tiron as a substrate for mushroom tyrosinase
Tiron (4,5-dihydroxy-1,3-benzene disulphonic acid) can serve as a substrate for mushroom tyrosinase with a Km value of 34 mM. The product(s) formed is yellow (λmax 435 nm) and is stable with time. An intermediate product, having significant absorbance at 3685 nm (probably tiron-o-quinone), was detected and the kinetics of its conversion to the final yellow product(s) was studied. Hydrogen peroxide (at 0.03-1.3 mM) accelerated the conversion of the intermediate compound to the yellow product(s). Tiron-semiquinone was detected by EPR spectroscopy during the initial phase of Tiron oxidation by mushroom tyrosinase. Maximum EPR signal intensity due to Tiron-semiquinone and the time required to reach maximum intensity were dependent on the amount of mushroom tyrosinase but not on the presence or absence of hydrogen peroxide (3.3 mM). In view of separate studies showing that the yellow product(s) is mainly a low M, polymerized Tiron-quinone, suggestions as to possible pathways by which such a product(s) is formed are discussed. © 1987.
Scientific Publication
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