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Loi, P., Department of Comparative Biomedical Sciences, Teramo University, Teramo, Italy
Matsukawa, K., Department of Comparative Biomedical Sciences, Teramo University, Teramo, Italy, National Institute of Livestock and Grassland Science, Osaka, Japan
Ptak, G., Department of Comparative Biomedical Sciences, Teramo University, Teramo, Italy
Clinton, M., Roslin Institute and Royal (Dick) School of Veterinary Studies, Edinburgh, United Kingdom
Fulka Jr., J., Institute of Animal Production, Prague, Czech Republic
Nathan, Y., Institute of Animal Science, Agricultural Research Organization, The Volcani Centre, Bet Dagan, Israel
Arav, A., Institute of Animal Science, Agricultural Research Organization, The Volcani Centre, Bet Dagan, Israel
The natural capacity of simple organisms to survive in a dehydrated state has long been exploited by man, with lyophylization the method of choice for the long term storage of bacterial and yeast cells. More recently, attempts have been made to apply this procedure to the long term storage of blood cells. However, despite significant progress, practical, application in a clinical setting is still some way off. Conversely, to date there are no reports of attempts to lyophilize nucleated somatic cells for possible downstream applications. Here we demonstrate that lyophilised somatic cells stored for 3 years at room temperature are able to direct embryonic development following injection into enucleated oocytes. These remarkable results demonstrate that alternative systems for the long-term storage of cell lines are now possible, and open unprecedented opportunities in the fields of biomedicine for conservation strategies. © 2008 Loi et al.
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Freeze-dried somatic cells direct embryonic development after nuclear transfer
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Loi, P., Department of Comparative Biomedical Sciences, Teramo University, Teramo, Italy
Matsukawa, K., Department of Comparative Biomedical Sciences, Teramo University, Teramo, Italy, National Institute of Livestock and Grassland Science, Osaka, Japan
Ptak, G., Department of Comparative Biomedical Sciences, Teramo University, Teramo, Italy
Clinton, M., Roslin Institute and Royal (Dick) School of Veterinary Studies, Edinburgh, United Kingdom
Fulka Jr., J., Institute of Animal Production, Prague, Czech Republic
Nathan, Y., Institute of Animal Science, Agricultural Research Organization, The Volcani Centre, Bet Dagan, Israel
Arav, A., Institute of Animal Science, Agricultural Research Organization, The Volcani Centre, Bet Dagan, Israel
Freeze-dried somatic cells direct embryonic development after nuclear transfer
The natural capacity of simple organisms to survive in a dehydrated state has long been exploited by man, with lyophylization the method of choice for the long term storage of bacterial and yeast cells. More recently, attempts have been made to apply this procedure to the long term storage of blood cells. However, despite significant progress, practical, application in a clinical setting is still some way off. Conversely, to date there are no reports of attempts to lyophilize nucleated somatic cells for possible downstream applications. Here we demonstrate that lyophilised somatic cells stored for 3 years at room temperature are able to direct embryonic development following injection into enucleated oocytes. These remarkable results demonstrate that alternative systems for the long-term storage of cell lines are now possible, and open unprecedented opportunities in the fields of biomedicine for conservation strategies. © 2008 Loi et al.
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