אסיף מאגר המחקר החקלאי

Electron spin resonance and spin-trapping studies of radiation damage in biologically significant molecules -(Radiation Physics and Chemistry)

Year:

1987

Source of publication :

Authors :

Volume :

30

Co-Authors:

Rosenthal, I., Division of Contaminants Chemistry, Center for Food Safety and Applied Nutrition, Food and Drug Administration, Washington, DC 20204, United States
Riesz, P., Division of Contaminants Chemistry, Center for Food Safety and Applied Nutrition, Food and Drug Administration, Washington, DC 20204, United States

Facilitators :

Link :

Abstract:

Recent electron spin resonance (ESR) and spin trapping studies of γ-radiation induced radicals in protein and nucleic acid constituents in the polycrystalline state and in aqueous solutions are reviewed. When complex mixtures of spin-adducts are generated, the identification of radicals is facilitated by combining spin trapping with high pressure liquid chromatography, using ESR as an in-line detector. For high molecular weight polypeptides and polynucleotides, enzymatic hydrolysis of the spin trapped macromolecular radicals appears to be a promising approach. Attempts to spin trap radicals inside γ-irradiated cells and whole animals are discussed.Recent electron spin resonance (ESR) and spin trapping studies of gamma -radiation induced radicals in protein and nuclei acid constituents in the polycrystalline state and in aqueous solutions are reviewed. When complex mixtures of spin-adducts are generated, the identification of radicals is facilitated by combining spin trapping with high pressure liquid chromatography, using ESR as an in-line detector. For high molecular weight polypeptides and polynucleotides, enzymatic hydrolysis of the spin trapped macromolecular radicals appears to be a promising approach. Attempts to spin trap radicals inside gamma -irradiated cells and whole animals are discussed.

Note:

Related Files :

Related Content

More details

You may also be interested in