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Plant phenolic volatiles inhibit quorum sensing in pectobacteria and reduce their virulence by potential binding to ExpI and ExpR proteins
Year:
2016
Source of publication :
Scientific Reports
Authors :
Lipsky, Alexander
;
.
Yedidia, Iris
;
.
Volume :
6
Co-Authors:
Joshi, J.R., Department of Plant Pathology and Microbiology, Otto Warburg Minerva Center for Agricultural Biotechnology, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel, Department of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Khazanov, N., Department of Chemistry, Bar-Ilan University, Ramat-Gan, Israel
Senderowitz, H., Department of Chemistry, Bar-Ilan University, Ramat-Gan, Israel
Burdman, S., Department of Plant Pathology and Microbiology, Otto Warburg Minerva Center for Agricultural Biotechnology, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Lipsky, A., Department of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Yedidia, I., Department of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
Quorum sensing (QS) is a population density-dependent regulatory system in bacteria that couples gene expression to cell density through accumulation of diffusible signaling molecules. Pectobacteria are causal agents of soft rot disease in a range of economically important crops. They rely on QS to coordinate their main virulence factor, production of plant cell wall degrading enzymes (PCWDEs). Plants have evolved an array of antimicrobial compounds to anticipate and cope with pathogens, of which essential oils (EOs) are widely recognized. Here, volatile EOs, carvacrol and eugenol, were shown to specifically interfere with QS, the master regulator of virulence in pectobacteria, resulting in strong inhibition of QS genes, biofilm formation and PCWDEs, thereby leading to impaired infection. Accumulation of the signal molecule N-acylhomoserine lactone declined upon treatment with EOs, suggesting direct interaction of EOs with either homoserine lactone synthase (ExpI) or with the regulatory protein (ExpR). Homology models of both proteins were constructed and docking simulations were performed to test the above hypotheses. The resulting binding modes and docking scores of carvacrol and eugenol support potential binding to ExpI/ExpR, with stronger interactions than previously known inhibitors of both proteins. The results demonstrate the potential involvement of phytochemicals in the control of Pectobacterium. © The Author(s) 2016.
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DOI :
10.1038/srep38126
Article number:
38126
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
27274
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:29
Scientific Publication
Plant phenolic volatiles inhibit quorum sensing in pectobacteria and reduce their virulence by potential binding to ExpI and ExpR proteins
6
Joshi, J.R., Department of Plant Pathology and Microbiology, Otto Warburg Minerva Center for Agricultural Biotechnology, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel, Department of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Khazanov, N., Department of Chemistry, Bar-Ilan University, Ramat-Gan, Israel
Senderowitz, H., Department of Chemistry, Bar-Ilan University, Ramat-Gan, Israel
Burdman, S., Department of Plant Pathology and Microbiology, Otto Warburg Minerva Center for Agricultural Biotechnology, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Lipsky, A., Department of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Yedidia, I., Department of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Plant phenolic volatiles inhibit quorum sensing in pectobacteria and reduce their virulence by potential binding to ExpI and ExpR proteins
Quorum sensing (QS) is a population density-dependent regulatory system in bacteria that couples gene expression to cell density through accumulation of diffusible signaling molecules. Pectobacteria are causal agents of soft rot disease in a range of economically important crops. They rely on QS to coordinate their main virulence factor, production of plant cell wall degrading enzymes (PCWDEs). Plants have evolved an array of antimicrobial compounds to anticipate and cope with pathogens, of which essential oils (EOs) are widely recognized. Here, volatile EOs, carvacrol and eugenol, were shown to specifically interfere with QS, the master regulator of virulence in pectobacteria, resulting in strong inhibition of QS genes, biofilm formation and PCWDEs, thereby leading to impaired infection. Accumulation of the signal molecule N-acylhomoserine lactone declined upon treatment with EOs, suggesting direct interaction of EOs with either homoserine lactone synthase (ExpI) or with the regulatory protein (ExpR). Homology models of both proteins were constructed and docking simulations were performed to test the above hypotheses. The resulting binding modes and docking scores of carvacrol and eugenol support potential binding to ExpI/ExpR, with stronger interactions than previously known inhibitors of both proteins. The results demonstrate the potential involvement of phytochemicals in the control of Pectobacterium. © The Author(s) 2016.
Scientific Publication
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