נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Ectopic expression of β-lactoglobulin/human serum albumin fusion genes in transgenic mice: hormonal regulation and in situ localization
Year:
1994
Source of publication :
Transgenic Research
Authors :
Barash, Itamar
;
.
Faerman, Alexander
;
.
Puzis, Raisa
;
.
Shani, Moshe
;
.
Volume :
3
Co-Authors:
Barash, I., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Faerman, A., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Ratovitsky, T., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Puzis, R., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Nathan, M., Rhone-Poulenc Rorer Central Research, 550 Arcola Rd., Collegeville, 19426-0107, PA, United States
Hurwitz, D.R., Rhone-Poulenc Rorer Central Research, 550 Arcola Rd., Collegeville, 19426-0107, PA, United States
Shani, M., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
141
To page:
151
(
Total pages:
11
)
Abstract:
We produced transgenic mice carrying the native sheep β-lactoglobulin (BLG) or fusion genes composed of the BLG promoter and human serum albumin (HSA) minigenes. BLG was expressed exclusively in the mammary glands of the virgin and lactating transgenic mice evaluated. In contrast, transgenic females carrying the BLG/HSA fusion constructs also expressed the HSA RNA ectopically in skeletal muscle, kidney, brain, spleen, salivary gland and skin. Ectopic expression of HSA RNA was detected only in strains that express the transgene in the mammary gland. There was no obvious correlation between the level of the HSA RNA expressed in the mammary gland and that found ectopically. In three transgenic strains analysed, the expression of HSA RNA in kidney and skeletal muscle increased during pregnancy and lactation, whereas in the brain HSA expression decreased during lactation in one of the strains. HSA protein was synthesized in skeletal muscle and skin of strain #23 and its level was higher in lactating mice compared with virgin mice. Expression of HSA was also analysed in males and was found to be more stringently controlled than in females of the same strains. In situ hybridization analyses localized the expressed transgene in the skin, kidney, brain and salivary glands of various transgenic strains. Distinct strain-specific and cell-type specific HSA expression patterns were observed in the skin. This is in contrast to the exclusive expression of the HSA transgene in epithelial cells surrounding the alveoli of the mammary gland. Taken together, these results suggest that the absence of sufficient mammary-specific regulatory elements in the BLG promoter sequences and/or the juxtaposition of the BLG promoter with the HSA coding sequences leads to novel tissue- and cell-specific expression in ectopic tissues of transgenic mice. © 1994 Chapman & Hall.
Note:
Related Files :
biosynthesis
Female
Genetics
lactation
Male
metabolism
mice
milk
pregnancy
sheep
Show More
Related Content
More details
DOI :
10.1007/BF01973981
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
27357
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:30
You may also be interested in
Scientific Publication
Ectopic expression of β-lactoglobulin/human serum albumin fusion genes in transgenic mice: hormonal regulation and in situ localization
3
Barash, I., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Faerman, A., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Ratovitsky, T., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Puzis, R., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Nathan, M., Rhone-Poulenc Rorer Central Research, 550 Arcola Rd., Collegeville, 19426-0107, PA, United States
Hurwitz, D.R., Rhone-Poulenc Rorer Central Research, 550 Arcola Rd., Collegeville, 19426-0107, PA, United States
Shani, M., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Ectopic expression of β-lactoglobulin/human serum albumin fusion genes in transgenic mice: hormonal regulation and in situ localization
We produced transgenic mice carrying the native sheep β-lactoglobulin (BLG) or fusion genes composed of the BLG promoter and human serum albumin (HSA) minigenes. BLG was expressed exclusively in the mammary glands of the virgin and lactating transgenic mice evaluated. In contrast, transgenic females carrying the BLG/HSA fusion constructs also expressed the HSA RNA ectopically in skeletal muscle, kidney, brain, spleen, salivary gland and skin. Ectopic expression of HSA RNA was detected only in strains that express the transgene in the mammary gland. There was no obvious correlation between the level of the HSA RNA expressed in the mammary gland and that found ectopically. In three transgenic strains analysed, the expression of HSA RNA in kidney and skeletal muscle increased during pregnancy and lactation, whereas in the brain HSA expression decreased during lactation in one of the strains. HSA protein was synthesized in skeletal muscle and skin of strain #23 and its level was higher in lactating mice compared with virgin mice. Expression of HSA was also analysed in males and was found to be more stringently controlled than in females of the same strains. In situ hybridization analyses localized the expressed transgene in the skin, kidney, brain and salivary glands of various transgenic strains. Distinct strain-specific and cell-type specific HSA expression patterns were observed in the skin. This is in contrast to the exclusive expression of the HSA transgene in epithelial cells surrounding the alveoli of the mammary gland. Taken together, these results suggest that the absence of sufficient mammary-specific regulatory elements in the BLG promoter sequences and/or the juxtaposition of the BLG promoter with the HSA coding sequences leads to novel tissue- and cell-specific expression in ectopic tissues of transgenic mice. © 1994 Chapman & Hall.
Scientific Publication
You may also be interested in