Co-Authors:
Icekson, I., Dept. of Fruit and Vegetable Storage, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Goldlust, A., Dept. of Fruit and Vegetable Storage, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Apelbaum, A., Dept. of Fruit and Vegetable Storage, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Abstract:
S-adenosyl-L-methionine decarboxylase was detected in crude extract from the plumular hook of etiolated pea seedlings. The enzyme showed a Km of 0.2mM for S-adenosyl-Lmethionine and a Vmax of 4.76 nmol/mg protein/h. It was not activated by putrescine or by Mg++ and was inhibited by methyl glyoxal-bis-guanyl hydrazone with an I50 of 1µM. Treatment of pea seedlings with ethylene resulted in a 75 % reduction of the enzyme activity with an I50 of 0.3 µl/1 and a maximal effect at 6µl/l. The reduction was observed within 1 hour after exposure to ethylene, reaching a maximum at 18 hours. Upon transferring the ethylenetreated plants to an ethylene-free atmosphere the enzyme gradually recovered from the inhibition, showing a complete recovery within 6 hours. Exposing pea seedlings to treatments that caused a reduction in the endogenous level of ethylene resulted in a 30–48 % increase in the enzyme activity. In view of the results of this study, the possibility that ethylene at the endogenous level might regulate S-adenosyl-L-methionine decarboxylase activity is discussed. © 1985, Gustav Fischer Verlag, Stuttgart. All rights reserved.
תפריט נגישות
ניגודיות עדינה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם
