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Identification of an Arabidopsis unknown small membrane protein targeted to mitochondria, chloroplasts, and peroxisomes
Year:
2009
Source of publication :
Protoplasma
Authors :
Abu-Abied, Mohamad
;
.
Avisar, Dror
;
.
Belausov, Eduard
;
.
Holdengreber, Vered
;
.
Sadot, Einat
;
.
Volume :
236
Co-Authors:

Abu-Abied, M., The Department of Ornamental Horticulture, The Institute of Plant Sciences, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Avisar, D., The Department of Ornamental Horticulture, The Institute of Plant Sciences, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Belausov, E., The Department of Ornamental Horticulture, The Institute of Plant Sciences, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Holdengreber, V., The Department of Ornamental Horticulture, The Institute of Plant Sciences, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Kam, Z., The Department of Molecular Biology of the Cell, The Weizmann Institute of Science, Rehovot 76100, Israel
Sadot, E., The Department of Ornamental Horticulture, The Institute of Plant Sciences, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel

Facilitators :
From page:
3
To page:
12
(
Total pages:
10
)
Abstract:
In a functional genomic screen performed by combining an Arabidopsis-yellow fluorescent protein (YFP)-fused complementary DNA (cDNA) library, rat fibroblasts as host and automatic microscopy, we found a short protein with a predictable trans-membrane domain encoded on chromosome 2. In rat fibroblasts, its pattern of distribution was to various organelle-like structures. From the databases, we learned that it has another family member in Arabidopsis and homologs in several other plants, Chlamydomonas and fungi, with a highly conserved N-terminal region. We named this protein from Arabidopsis short membrane protein (SMP) 2. No SMP homologs were found in mammalian sequence databases. When the full-length cDNAs of SMP2 was fused to YFP under the 35S promoter, comparable distribution was observed in Nicotiana benthamiana leaves, suggesting an unknown, evolutionarily conserved localization signal. Similar localization was observed when SMP2 was expressed in N. benthamiana leaves under the control of its own 5′ regulatory sequences. Colocalization studies with green fluorescent protein and red fluorescent protein chimeras revealed its colocalization with chloroplasts, peroxisomes, and mitochondria. No localization of SMP2 was observed in the Golgi. Immunostaining with specific antibodies corroborated the SMP2 localization to the three organelles. © Springer-Verlag 2009.
Note:
Related Files :
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arabidopsis
fungi
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metabolism
molecular genetics
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More details
DOI :
10.1007/s00709-009-0038-7
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
27458
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:31
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Scientific Publication
Identification of an Arabidopsis unknown small membrane protein targeted to mitochondria, chloroplasts, and peroxisomes
236

Abu-Abied, M., The Department of Ornamental Horticulture, The Institute of Plant Sciences, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Avisar, D., The Department of Ornamental Horticulture, The Institute of Plant Sciences, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Belausov, E., The Department of Ornamental Horticulture, The Institute of Plant Sciences, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Holdengreber, V., The Department of Ornamental Horticulture, The Institute of Plant Sciences, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Kam, Z., The Department of Molecular Biology of the Cell, The Weizmann Institute of Science, Rehovot 76100, Israel
Sadot, E., The Department of Ornamental Horticulture, The Institute of Plant Sciences, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel

Identification of an Arabidopsis unknown small membrane protein targeted to mitochondria, chloroplasts, and peroxisomes
In a functional genomic screen performed by combining an Arabidopsis-yellow fluorescent protein (YFP)-fused complementary DNA (cDNA) library, rat fibroblasts as host and automatic microscopy, we found a short protein with a predictable trans-membrane domain encoded on chromosome 2. In rat fibroblasts, its pattern of distribution was to various organelle-like structures. From the databases, we learned that it has another family member in Arabidopsis and homologs in several other plants, Chlamydomonas and fungi, with a highly conserved N-terminal region. We named this protein from Arabidopsis short membrane protein (SMP) 2. No SMP homologs were found in mammalian sequence databases. When the full-length cDNAs of SMP2 was fused to YFP under the 35S promoter, comparable distribution was observed in Nicotiana benthamiana leaves, suggesting an unknown, evolutionarily conserved localization signal. Similar localization was observed when SMP2 was expressed in N. benthamiana leaves under the control of its own 5′ regulatory sequences. Colocalization studies with green fluorescent protein and red fluorescent protein chimeras revealed its colocalization with chloroplasts, peroxisomes, and mitochondria. No localization of SMP2 was observed in the Golgi. Immunostaining with specific antibodies corroborated the SMP2 localization to the three organelles. © Springer-Verlag 2009.
Scientific Publication
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