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Characterization of hydroxamate siderophores and siderophore-mediated iron uptake in Gaeumannomyces graminis var. tritici
Year:
1990
Authors :
Solel, Zvi
;
.
Volume :
37
Co-Authors:
Dori, S., Department of Botany, Tel-Aviv University, Tel Aviv, 69978, Israel
Solel, Z., Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Kashman, Y., Department of Chemistry, Tel-Aviv University, Tel Aviv, 69978, Israel
Barash, I., Department of Botany, Tel-Aviv University, Tel Aviv, 69978, Israel, Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
95
To page:
106
(
Total pages:
12
)
Abstract:
Under iron-deficient conditions, Gaeumannomyces graminis var. tritici (Ggt) produces dimerum acid and coprogen B as well as traces of two other unidentified hydroxamate siderophores. The production of coprogen B decreased and of dimerum acid increased with the rise in pH of the growth medium. While iron concentration of 3 μm completely repressed siderophore secretion, a low iron concentration (0·06 μm) was necessary for maximal production. Siderophore-mediated iron uptake for dimerum acid and coprogen B exhibited active transport and was optimum at pH 4·5-5 and 30 °C. Michaelis kinetics with apparent Kms of 6·8 and 11 μm, and Vmax of 0·26 and 0·54 nmoles mg-1 min-1 was obtained for dimerum acid and coprogen B, respectively. While Ggt could utilize iron effectively from siderophores produced by other fungi, utilization of iron from pseudobactin, ferrioxamine B, EDTA and EDDHA was ineffective. Competition studies between dimerum acid and various siderophores suggest a common transport system with coprogen B, rhodotorulic acid and ferrichrome but not with fusigen and triacetylfusigen. Wheat seedlings were capable of utilizing iron efficiently from dimerum acid and coprogen B. The possible influence of the pathogen's siderophores on its survival and virulence is discussed. © 1990.
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DOI :
10.1016/0885-5765(90)90002-F
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
27494
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:31
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Scientific Publication
Characterization of hydroxamate siderophores and siderophore-mediated iron uptake in Gaeumannomyces graminis var. tritici
37
Dori, S., Department of Botany, Tel-Aviv University, Tel Aviv, 69978, Israel
Solel, Z., Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Kashman, Y., Department of Chemistry, Tel-Aviv University, Tel Aviv, 69978, Israel
Barash, I., Department of Botany, Tel-Aviv University, Tel Aviv, 69978, Israel, Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Characterization of hydroxamate siderophores and siderophore-mediated iron uptake in Gaeumannomyces graminis var. tritici
Under iron-deficient conditions, Gaeumannomyces graminis var. tritici (Ggt) produces dimerum acid and coprogen B as well as traces of two other unidentified hydroxamate siderophores. The production of coprogen B decreased and of dimerum acid increased with the rise in pH of the growth medium. While iron concentration of 3 μm completely repressed siderophore secretion, a low iron concentration (0·06 μm) was necessary for maximal production. Siderophore-mediated iron uptake for dimerum acid and coprogen B exhibited active transport and was optimum at pH 4·5-5 and 30 °C. Michaelis kinetics with apparent Kms of 6·8 and 11 μm, and Vmax of 0·26 and 0·54 nmoles mg-1 min-1 was obtained for dimerum acid and coprogen B, respectively. While Ggt could utilize iron effectively from siderophores produced by other fungi, utilization of iron from pseudobactin, ferrioxamine B, EDTA and EDDHA was ineffective. Competition studies between dimerum acid and various siderophores suggest a common transport system with coprogen B, rhodotorulic acid and ferrichrome but not with fusigen and triacetylfusigen. Wheat seedlings were capable of utilizing iron efficiently from dimerum acid and coprogen B. The possible influence of the pathogen's siderophores on its survival and virulence is discussed. © 1990.
Scientific Publication
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