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The effect of high temperature and high atmospheric Co2 on carbohydrate changes in bell pepper (Capsicum annuum) pollen in relation to its germination
Year:
2001
Source of publication :
Physiologia Plantarum
Authors :
Aloni, Benyamin
;
.
Karni, Leah
;
.
Volume :
112
Co-Authors:
Aloni, B., Department of Vegetable Crops, Institute of Field and Garden Crops, Volcani Center, Bet Dagan, 50250, Israel
Peet, M., Department of Horticulture, Kilgore Hall, North Carolina State University, Raleigh, NC 27695-7609, United States
Pharr, M., Department of Horticulture, Kilgore Hall, North Carolina State University, Raleigh, NC 27695-7609, United States
Karni, L., Department of Vegetable Crops, Institute of Field and Garden Crops, Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
505
To page:
512
(
Total pages:
8
)
Abstract:
Pollen viability and germination are known to be sensitive to high temperature (HT). However, the mode by which high temperature impairs pollen functioning is not yet clear. In the present study, we investigated the effect of high temperature on changes occurring in carbohydrate of bell pepper (Capsicum annuum L. cv. Mazurka) pollen in order to find possible relations between these changes and pollen germination under heat stress. When pepper plants were maintained under a moderate HT regime (32/26°C, day/night) for 8 days before flowers have reached anthesis, pollen count at anthesis was similar to that found in plants grown under normal temperatures (NT 28/22°C). However, the in vitro germination, carried out at 25°C, of pollen from HT plants was greatly reduced. This effect matched the marked reduction in the number of seeds per fruit in the HT plants. Maintaining the plants at high air CO2 concentration (800 μmol mol-1 air) in both temperature treatments did not affect the in vitro germination of pollen from NT plants, but restored germination to near the normal level in pollen from HT plants. Under NT conditions, starch, which was negligible in pollen at meiosis (8 days before anthesis, A - 8) started to accumulate at A - 4 and continued to accumulate until A - 2. From that stage until anthesis, starch was rapidly degraded. On the other hand, sucrose concentration rose from stage A - 4 until anthesis. Acid invertase (EC 3.2.1.26) activity rose parallel with the increase of sucrose. In pollen from HT plants, sucrose and starch concentrations were significantly higher at A - 1 pollen than in that of NT plants. Under high CO2 conditions, the sucrose concentration in the pollen of HT plants was reduced to levels similar to those in NT pollen. In accordance with the higher sucrose concentration in HT pollen, the acid invertase activity in these pollen grains was lower than in NT pollen. The results suggest that the higher concentrations of sucrose and starch in the pollen grains of HT plants may result from reduction in their metabolism under heat stress. Elevated CO2 concentration, presumably by increasing assimilate availability to the pollen grain, may alleviate the inhibition of sucrose and starch metabolism, thereby increasing their utilization for pollen germination under the HT stress. Acid invertase may have a regulatory role in this system.
Note:
Related Files :
Capsicum annuum
carbohydrates
enzymes
flowering
Fruits
germination
heat stress
metabolism
Show More
Related Content
More details
DOI :
10.1034/j.1399-3054.2001.1120407.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
27530
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:31
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Scientific Publication
The effect of high temperature and high atmospheric Co2 on carbohydrate changes in bell pepper (Capsicum annuum) pollen in relation to its germination
112
Aloni, B., Department of Vegetable Crops, Institute of Field and Garden Crops, Volcani Center, Bet Dagan, 50250, Israel
Peet, M., Department of Horticulture, Kilgore Hall, North Carolina State University, Raleigh, NC 27695-7609, United States
Pharr, M., Department of Horticulture, Kilgore Hall, North Carolina State University, Raleigh, NC 27695-7609, United States
Karni, L., Department of Vegetable Crops, Institute of Field and Garden Crops, Volcani Center, Bet Dagan, 50250, Israel
The effect of high temperature and high atmospheric Co2 on carbohydrate changes in bell pepper (Capsicum annuum) pollen in relation to its germination
Pollen viability and germination are known to be sensitive to high temperature (HT). However, the mode by which high temperature impairs pollen functioning is not yet clear. In the present study, we investigated the effect of high temperature on changes occurring in carbohydrate of bell pepper (Capsicum annuum L. cv. Mazurka) pollen in order to find possible relations between these changes and pollen germination under heat stress. When pepper plants were maintained under a moderate HT regime (32/26°C, day/night) for 8 days before flowers have reached anthesis, pollen count at anthesis was similar to that found in plants grown under normal temperatures (NT 28/22°C). However, the in vitro germination, carried out at 25°C, of pollen from HT plants was greatly reduced. This effect matched the marked reduction in the number of seeds per fruit in the HT plants. Maintaining the plants at high air CO2 concentration (800 μmol mol-1 air) in both temperature treatments did not affect the in vitro germination of pollen from NT plants, but restored germination to near the normal level in pollen from HT plants. Under NT conditions, starch, which was negligible in pollen at meiosis (8 days before anthesis, A - 8) started to accumulate at A - 4 and continued to accumulate until A - 2. From that stage until anthesis, starch was rapidly degraded. On the other hand, sucrose concentration rose from stage A - 4 until anthesis. Acid invertase (EC 3.2.1.26) activity rose parallel with the increase of sucrose. In pollen from HT plants, sucrose and starch concentrations were significantly higher at A - 1 pollen than in that of NT plants. Under high CO2 conditions, the sucrose concentration in the pollen of HT plants was reduced to levels similar to those in NT pollen. In accordance with the higher sucrose concentration in HT pollen, the acid invertase activity in these pollen grains was lower than in NT pollen. The results suggest that the higher concentrations of sucrose and starch in the pollen grains of HT plants may result from reduction in their metabolism under heat stress. Elevated CO2 concentration, presumably by increasing assimilate availability to the pollen grain, may alleviate the inhibition of sucrose and starch metabolism, thereby increasing their utilization for pollen germination under the HT stress. Acid invertase may have a regulatory role in this system.
Scientific Publication
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