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A method for activity staining of peroxidase and β-1,3-glucanase isozymes in polyacrylamide electrophoresis gels
Year:
1994
Source of publication :
Analytical Biochemistry
Authors :
Shimoni, Moshe
;
.
Volume :
220
Co-Authors:
Shimoni, M., Division of Plant Pathology, Agricultural Research Organization, Newe Ya'ar Research Center, Haifa Post 31 999, Israel
Facilitators :
From page:
36
To page:
38
(
Total pages:
3
)
Abstract:
A procedure was developed for activity staining of peroxidase and β-1,3- glucanase isozymes (a double staining procedure) in the same polyacrylamide gels using 3-amino-9-ethylcarbazol for peroxidase staining and laminarine and 2,3,5-triphenyltetrazolium chloride for glucanase staining. The procedure was used to detect peroxidase and β-1,3-glucanase in different corn lines using plant leaf extract.
Note:
Related Files :
1,3 beta glucanase
Electrophoresis, Polyacrylamide Gel
enzyme activity
peroxidase
Zea mays
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Related Content
More details
DOI :
10.1006/abio.1994.1295
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
27578
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:32
Scientific Publication
A method for activity staining of peroxidase and β-1,3-glucanase isozymes in polyacrylamide electrophoresis gels
220
Shimoni, M., Division of Plant Pathology, Agricultural Research Organization, Newe Ya'ar Research Center, Haifa Post 31 999, Israel
A method for activity staining of peroxidase and β-1,3-glucanase isozymes in polyacrylamide electrophoresis gels
A procedure was developed for activity staining of peroxidase and β-1,3- glucanase isozymes (a double staining procedure) in the same polyacrylamide gels using 3-amino-9-ethylcarbazol for peroxidase staining and laminarine and 2,3,5-triphenyltetrazolium chloride for glucanase staining. The procedure was used to detect peroxidase and β-1,3-glucanase in different corn lines using plant leaf extract.
Scientific Publication
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