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Metabolomics in melon: A new opportunity for aroma analysis
Year:
2014
Source of publication :
Phytochemistry
Authors :
Burger, Joseph
;
.
Schaffer, Arthur
;
.
Volume :
99
Co-Authors:

Allwood, J.W., School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom, School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
Cheung, W., School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom
Xu, Y., School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom
Mumm, R., Plant Research International, P.O. Box 16, 6700 AA Wageningen, Netherlands, Netherlands Metabolomics Centre, Einsteinweg 55, 2333 CC Leiden, Netherlands
De Vos, R.C.H., Plant Research International, P.O. Box 16, 6700 AA Wageningen, Netherlands, Netherlands Metabolomics Centre, Einsteinweg 55, 2333 CC Leiden, Netherlands, Centre for BioSystems Genomics, P.O. Box 98, 6700AB Wageningen, Netherlands
Deborde, C., INRA, UMR1332 Biologie du Fruit et Pathologie, INRA - Université de Bordeaux, F-33140 Villenave d'Ornon, France, Metabolome Facility of Bordeaux Functional Genomics Centre, Centre INRA de Bordeaux, IBVM, F-33140 Villenave d'Ornon, France
Biais, B., INRA, UMR1332 Biologie du Fruit et Pathologie, INRA - Université de Bordeaux, F-33140 Villenave d'Ornon, France
Maucourt, M., Metabolome Facility of Bordeaux Functional Genomics Centre, Centre INRA de Bordeaux, IBVM, F-33140 Villenave d'Ornon, France, Université de Bordeaux, UMR1332 Biologie du Fruit et Pathologie, Centre INRA de Bordeaux, F-33140 Villenave d'Ornon, France
Burger, Y., Agricultural Research Organisation (ARO), Volcani Center, Bet Dagan 50250, Israel
Schaffer, A.A., Agricultural Research Organisation (ARO), Volcani Center, Bet Dagan 50250, Israel
Rolin, D., Metabolome Facility of Bordeaux Functional Genomics Centre, Centre INRA de Bordeaux, IBVM, F-33140 Villenave d'Ornon, France, Université de Bordeaux, UMR1332 Biologie du Fruit et Pathologie, Centre INRA de Bordeaux, F-33140 Villenave d'Ornon, France
Moing, A., INRA, UMR1332 Biologie du Fruit et Pathologie, INRA - Université de Bordeaux, F-33140 Villenave d'Ornon, France, Metabolome Facility of Bordeaux Functional Genomics Centre, Centre INRA de Bordeaux, IBVM, F-33140 Villenave d'Ornon, France
Hall, R.D., Plant Research International, P.O. Box 16, 6700 AA Wageningen, Netherlands, Netherlands Metabolomics Centre, Einsteinweg 55, 2333 CC Leiden, Netherlands, Centre for BioSystems Genomics, P.O. Box 98, 6700AB Wageningen, Netherlands
Goodacre, R., School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom, Manchester Centre for Integrative Systems Biology, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom

Facilitators :
From page:
61
To page:
72
(
Total pages:
12
)
Abstract:
Cucumis melo fruit is highly valued for its sweet and refreshing flesh, however the flavour and value are also highly influenced by aroma as dictated by volatile organic compounds (VOCs). A simple and robust method of sampling VOCs on polydimethylsiloxane (PDMS) has been developed. Contrasting cultivars of C. melo subspecies melo were investigated at commercial maturity: three cultivars of var. Cantalupensis group Charentais (cv. Cézanne, Escrito, and Dalton) known to exhibit differences in ripening behaviour and shelf-life, as well as one cultivar of var. Cantalupensis group Ha'Ogan (cv. Noy Yisre'el) and one non-climacteric cultivar of var. Inodorus (cv. Tam Dew). The melon cultivar selection was based upon fruits exhibiting clear differences (cv. Noy Yisre'el and Tam Dew) and similarities (cv. Cézanne, Escrito, and Dalton) in flavour. In total, 58 VOCs were detected by thermal desorption (TD)-GC-MS which permitted the discrimination of each cultivar via Principal component analysis (PCA). PCA indicated a reduction in VOCs in the non-climacteric cv. Tam Dew compared to the four Cantalupensis cultivars. Within the group Charentais melons, the differences between the short, mid and long shelf-life cultivars were considerable. 1H NMR analysis led to the quantification of 12 core amino acids, their levels were 3-10-fold greater in the Charentais melons, although they were reduced in the highly fragrant cv. Cézanne, indicating their role as VOC precursors. This study along with comparisons to more traditional labour intensive solid phase micro-extraction (SPME) GC-MS VOC profiling data has indicated that the high-throughput PDMS method is of great potential for the assessment of melon aroma and quality. © 2013 Elsevier Ltd. All rights rese.
Note:
Related Files :
chemistry
Cucumis melo
metabolism
Metabolomics
odor
odors
Volatile organic compound (VOC)
Volatile organic compounds
Show More
Related Content
More details
DOI :
10.1016/j.phytochem.2013.12.010
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
27673
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:33
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Scientific Publication
Metabolomics in melon: A new opportunity for aroma analysis
99

Allwood, J.W., School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom, School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
Cheung, W., School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom
Xu, Y., School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom
Mumm, R., Plant Research International, P.O. Box 16, 6700 AA Wageningen, Netherlands, Netherlands Metabolomics Centre, Einsteinweg 55, 2333 CC Leiden, Netherlands
De Vos, R.C.H., Plant Research International, P.O. Box 16, 6700 AA Wageningen, Netherlands, Netherlands Metabolomics Centre, Einsteinweg 55, 2333 CC Leiden, Netherlands, Centre for BioSystems Genomics, P.O. Box 98, 6700AB Wageningen, Netherlands
Deborde, C., INRA, UMR1332 Biologie du Fruit et Pathologie, INRA - Université de Bordeaux, F-33140 Villenave d'Ornon, France, Metabolome Facility of Bordeaux Functional Genomics Centre, Centre INRA de Bordeaux, IBVM, F-33140 Villenave d'Ornon, France
Biais, B., INRA, UMR1332 Biologie du Fruit et Pathologie, INRA - Université de Bordeaux, F-33140 Villenave d'Ornon, France
Maucourt, M., Metabolome Facility of Bordeaux Functional Genomics Centre, Centre INRA de Bordeaux, IBVM, F-33140 Villenave d'Ornon, France, Université de Bordeaux, UMR1332 Biologie du Fruit et Pathologie, Centre INRA de Bordeaux, F-33140 Villenave d'Ornon, France
Burger, Y., Agricultural Research Organisation (ARO), Volcani Center, Bet Dagan 50250, Israel
Schaffer, A.A., Agricultural Research Organisation (ARO), Volcani Center, Bet Dagan 50250, Israel
Rolin, D., Metabolome Facility of Bordeaux Functional Genomics Centre, Centre INRA de Bordeaux, IBVM, F-33140 Villenave d'Ornon, France, Université de Bordeaux, UMR1332 Biologie du Fruit et Pathologie, Centre INRA de Bordeaux, F-33140 Villenave d'Ornon, France
Moing, A., INRA, UMR1332 Biologie du Fruit et Pathologie, INRA - Université de Bordeaux, F-33140 Villenave d'Ornon, France, Metabolome Facility of Bordeaux Functional Genomics Centre, Centre INRA de Bordeaux, IBVM, F-33140 Villenave d'Ornon, France
Hall, R.D., Plant Research International, P.O. Box 16, 6700 AA Wageningen, Netherlands, Netherlands Metabolomics Centre, Einsteinweg 55, 2333 CC Leiden, Netherlands, Centre for BioSystems Genomics, P.O. Box 98, 6700AB Wageningen, Netherlands
Goodacre, R., School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom, Manchester Centre for Integrative Systems Biology, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom

Metabolomics in melon: A new opportunity for aroma analysis
Cucumis melo fruit is highly valued for its sweet and refreshing flesh, however the flavour and value are also highly influenced by aroma as dictated by volatile organic compounds (VOCs). A simple and robust method of sampling VOCs on polydimethylsiloxane (PDMS) has been developed. Contrasting cultivars of C. melo subspecies melo were investigated at commercial maturity: three cultivars of var. Cantalupensis group Charentais (cv. Cézanne, Escrito, and Dalton) known to exhibit differences in ripening behaviour and shelf-life, as well as one cultivar of var. Cantalupensis group Ha'Ogan (cv. Noy Yisre'el) and one non-climacteric cultivar of var. Inodorus (cv. Tam Dew). The melon cultivar selection was based upon fruits exhibiting clear differences (cv. Noy Yisre'el and Tam Dew) and similarities (cv. Cézanne, Escrito, and Dalton) in flavour. In total, 58 VOCs were detected by thermal desorption (TD)-GC-MS which permitted the discrimination of each cultivar via Principal component analysis (PCA). PCA indicated a reduction in VOCs in the non-climacteric cv. Tam Dew compared to the four Cantalupensis cultivars. Within the group Charentais melons, the differences between the short, mid and long shelf-life cultivars were considerable. 1H NMR analysis led to the quantification of 12 core amino acids, their levels were 3-10-fold greater in the Charentais melons, although they were reduced in the highly fragrant cv. Cézanne, indicating their role as VOC precursors. This study along with comparisons to more traditional labour intensive solid phase micro-extraction (SPME) GC-MS VOC profiling data has indicated that the high-throughput PDMS method is of great potential for the assessment of melon aroma and quality. © 2013 Elsevier Ltd. All rights rese.
Scientific Publication
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