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An efficient method for the synthesis and purification of trans-[14C]geranylgeranyl pyrophosphate
Year:
1979
Source of publication :
Analytical Biochemistry
Authors :
Gafni, Yedidya
;
.
Volume :
92
Co-Authors:
Gafni, Y., Department of Biochemistry, The George S. Wise Center for Life Sciences, Tel-Aviv University, Tel-Aviv, Israel
Shechter, I., Department of Biochemistry, The George S. Wise Center for Life Sciences, Tel-Aviv University, Tel-Aviv, Israel
Facilitators :
From page:
248
To page:
252
(
Total pages:
5
)
Abstract:
A simple procedure for the preparation and purification of trans-[14C]geranylgeranyl pyrophosphate from dl-[14C]mevalonate is described. This procedure involves biosynthesis of trans-[14C]geranylgeraniol from dl-[14C]mevalonate by cell-free extracts of the fungus Gibberella fujikuroi and chemical synthesis of trans-[14C]geranylgeranyl pyrophosphate from trans-[14C]geranylgeraniol. The purification of trans-[14C]geranylgeraniol is achieved by chromatography on a silver nitrate-impregnated silica gel column. The pyrophosphate ester is purified on a silica gel column. © 1979.
Note:
Related Files :
biosynthesis
Chromatography, Gel
Diterpenes
geranyl pyrophosphate
Gibberella
mevalonic acid c 14
Organophosphorus Compounds
Show More
Related Content
More details
DOI :
10.1016/0003-2697(79)90653-5
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
27680
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:33
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Scientific Publication
An efficient method for the synthesis and purification of trans-[14C]geranylgeranyl pyrophosphate
92
Gafni, Y., Department of Biochemistry, The George S. Wise Center for Life Sciences, Tel-Aviv University, Tel-Aviv, Israel
Shechter, I., Department of Biochemistry, The George S. Wise Center for Life Sciences, Tel-Aviv University, Tel-Aviv, Israel
An efficient method for the synthesis and purification of trans-[14C]geranylgeranyl pyrophosphate
A simple procedure for the preparation and purification of trans-[14C]geranylgeranyl pyrophosphate from dl-[14C]mevalonate is described. This procedure involves biosynthesis of trans-[14C]geranylgeraniol from dl-[14C]mevalonate by cell-free extracts of the fungus Gibberella fujikuroi and chemical synthesis of trans-[14C]geranylgeranyl pyrophosphate from trans-[14C]geranylgeraniol. The purification of trans-[14C]geranylgeraniol is achieved by chromatography on a silver nitrate-impregnated silica gel column. The pyrophosphate ester is purified on a silica gel column. © 1979.
Scientific Publication
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