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Examination of the abscission-associated transcriptomes for soybean, tomato, and arabidopsis highlights the conserved biosynthesis of an extensible extracellular matrix and boundary layer
Year:
2015
Source of publication :
Frontiers in Plant Science
Authors :
Meir, Shimon
;
.
Philosoph-Hadas, Sonia
;
.
Volume :
6
Co-Authors:
Kim, J., Soybean Genomics and Improvement Lab, Agricultural Research Service, United States Department of Agriculture, Beitsviiie, MD, United States
Sundaresan, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Voicani Center, Bet-Dagan, Israel, The Robert H. Smith Facuity of Agricuiture, Food and Environment, The Hebrew University of Jerusaiem, Rehovot, Israel
Philosoph-Hadas, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Voicani Center, Bet-Dagan, Israel
Yang, R., Soybean Genomics and Improvement Lab, Agricultural Research Service, United States Department of Agriculture, Beitsviiie, MD, United States
Meir, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Voicani Center, Bet-Dagan, Israel
Tucker, M.L., Soybean Genomics and Improvement Lab, Agricultural Research Service, United States Department of Agriculture, Beitsviiie, MD, United States
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
Abscission zone (AZ) development and the progression of abscission (detachment of plant organs) have been roughly separated into four stages: first, AZ differentiation; second, competence to respond to abscission signals; third, activation of abscission; and fourth, formation of a protective layer and post-abscission trans-differentiation. Stage three, activation of abscission, is when changes in the cell wall and extracellular matrix occur to support successful organ separation. Most abscission research has focused on gene expression for enzymes that disassemble the cell wall within the AZ and changes in phytohormones and other signaling events that regulate their expression. Here, transcriptome data for soybean, tomato and Arabidopsis were examined and compared with a focus not only on genes associated with disassembly of the cell wall but also on gene expression linked to the biosynthesis of a new extracellular matrix. AZ-specific up-regulation of genes associated with cell wall disassembly including cellulases (beta-1,4-endoglucanases, CELs), polygalacturonases (PGs), and expansins (EXPs) were much as expected; however, curiously, changes in expression of xyloglucan endotransglucosylase/hydrolases (XTHs) were not AZ-specific in soybean. Unexpectedly, we identified an early increase in the expression of genes underlying the synthesis of a waxy-like cuticle. Based on the expression data, we propose that the early up-regulation of an abundance of small pathogenesis-related (PR) genes is more closely linked to structural changes in the extracellular matrix of separating cells than an enzymatic role in pathogen resistance. Furthermore, these observations led us to propose that, in addition to cell wall loosening enzymes, abscission requires (or is enhanced by) biosynthesis and secretion of small proteins (15-25 kDa) and waxes that form an extensible extracellular matrix and boundary layer on the surface of separating cells. The synthesis of the boundary layer precedes what is typically associated with the post-abscission synthesis of a protective scar over the fracture plane. This modification in the abscission model is discussed in regard to how it influences our interpretation of the role of multiple abscission signals. © 2015, Frontiers Research Foundation. All rights reserved
Note:
Related Files :
abscission
arabidopsis
Cuticle biosynthesis
ethylene
IDA
soybean
tomato
transcriptome
Show More
Related Content
More details
DOI :
10.3389/fpls.2015.01109
Article number:
1109
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
27702
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:33
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Examination of the abscission-associated transcriptomes for soybean, tomato, and arabidopsis highlights the conserved biosynthesis of an extensible extracellular matrix and boundary layer
6
Kim, J., Soybean Genomics and Improvement Lab, Agricultural Research Service, United States Department of Agriculture, Beitsviiie, MD, United States
Sundaresan, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Voicani Center, Bet-Dagan, Israel, The Robert H. Smith Facuity of Agricuiture, Food and Environment, The Hebrew University of Jerusaiem, Rehovot, Israel
Philosoph-Hadas, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Voicani Center, Bet-Dagan, Israel
Yang, R., Soybean Genomics and Improvement Lab, Agricultural Research Service, United States Department of Agriculture, Beitsviiie, MD, United States
Meir, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Voicani Center, Bet-Dagan, Israel
Tucker, M.L., Soybean Genomics and Improvement Lab, Agricultural Research Service, United States Department of Agriculture, Beitsviiie, MD, United States
Examination of the abscission-associated transcriptomes for soybean, tomato, and arabidopsis highlights the conserved biosynthesis of an extensible extracellular matrix and boundary layer
Abscission zone (AZ) development and the progression of abscission (detachment of plant organs) have been roughly separated into four stages: first, AZ differentiation; second, competence to respond to abscission signals; third, activation of abscission; and fourth, formation of a protective layer and post-abscission trans-differentiation. Stage three, activation of abscission, is when changes in the cell wall and extracellular matrix occur to support successful organ separation. Most abscission research has focused on gene expression for enzymes that disassemble the cell wall within the AZ and changes in phytohormones and other signaling events that regulate their expression. Here, transcriptome data for soybean, tomato and Arabidopsis were examined and compared with a focus not only on genes associated with disassembly of the cell wall but also on gene expression linked to the biosynthesis of a new extracellular matrix. AZ-specific up-regulation of genes associated with cell wall disassembly including cellulases (beta-1,4-endoglucanases, CELs), polygalacturonases (PGs), and expansins (EXPs) were much as expected; however, curiously, changes in expression of xyloglucan endotransglucosylase/hydrolases (XTHs) were not AZ-specific in soybean. Unexpectedly, we identified an early increase in the expression of genes underlying the synthesis of a waxy-like cuticle. Based on the expression data, we propose that the early up-regulation of an abundance of small pathogenesis-related (PR) genes is more closely linked to structural changes in the extracellular matrix of separating cells than an enzymatic role in pathogen resistance. Furthermore, these observations led us to propose that, in addition to cell wall loosening enzymes, abscission requires (or is enhanced by) biosynthesis and secretion of small proteins (15-25 kDa) and waxes that form an extensible extracellular matrix and boundary layer on the surface of separating cells. The synthesis of the boundary layer precedes what is typically associated with the post-abscission synthesis of a protective scar over the fracture plane. This modification in the abscission model is discussed in regard to how it influences our interpretation of the role of multiple abscission signals. © 2015, Frontiers Research Foundation. All rights reserved
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