Co-Authors:
Greer, C.W., Department of Microbiology and Immunology, McGill University, 3775 University Street, Montreal, Que. H3A 2B4, Canada
Shomer, I., Department of Microbiology and Immunology, McGill University, 3775 University Street, Montreal, Que. H3A 2B4, Canada, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet-Dagan, 50520, Israel
Goldstein, M.E., Department of Microbiology and Immunology, McGill University, 3775 University Street, Montreal, Que. H3A 2B4, Canada, Department of Biology, McGill University, Montreal, Que. H3A 1B1, Canada
Yaphe, W., Department of Microbiology and Immunology, McGill University, 3775 University Street, Montreal, Que. H3A 2B4, Canada
Abstract:
Carrageenan extracted from Hypnea musciformis was analyzed by using κ- and ι-carrageenanases and 13C-n.m.r. spectroscopy. κ-Carrageenanase caused extensive degradation of the polysaccharide, with the production of low-molecular-weight oligosaccharides of κ-carrageenan. Limited degradation of the polymer occurred with ι-carrageenanase, with the virtual absence of low-molecular-weight oligosaccharides. The native carrageenan had a 13C-n.m.r. spectrum characteristic for κ-carrageenan, but, after treatment with κ-carrageenanase, resonance peaks for ι-carrageenan were observed in the spectrum of the enzyme-resistant fraction. The carrageenan from H. musciformis is a hybrid, consisting mainly of κ-type repeating units, with minor proportions of ⇔-type repeating units and other components. © 1984.
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