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Towards the first linkage map of the Didymella rabiei genome
Year:
2002
Source of publication :
Phytoparasitica
Authors :
Shtienberg, Dan
;
.
Volume :
30
Co-Authors:
Lichtenzveig, J., Dept. of Fld. Crops, Veg./Genet., Hebrew University of Jerusalem, Faculty of Agricultural Sciences, Rehovot 76100, Israel
Winter, P., Dept. of Fld. Crops, Veg./Genet., Hebrew University of Jerusalem, Faculty of Agricultural Sciences, Rehovot 76100, Israel
Abbo, S., Dept. of Fld. Crops, Veg./Genet., Hebrew University of Jerusalem, Faculty of Agricultural Sciences, Rehovot 76100, Israel
Shtienberg, D., Dept. of Fld. Crops, Veg./Genet., Hebrew University of Jerusalem, Faculty of Agricultural Sciences, Rehovot 76100, Israel
Kaiser, W.J., Dept. of Fld. Crops, Veg./Genet., Hebrew University of Jerusalem, Faculty of Agricultural Sciences, Rehovot 76100, Israel
Kahl, G., Dept. of Fld. Crops, Veg./Genet., Hebrew University of Jerusalem, Faculty of Agricultural Sciences, Rehovot 76100, Israel
Facilitators :
From page:
467
To page:
472
(
Total pages:
6
)
Abstract:
A genetic map was developed for the ascomycete Didymella rabiei (Kovachevski) v. Arx (anamorph: Ascochyta rabiei Pass. Labr.), the causal agent of Ascochyta blight in chickpea (Cicer arietinum L.). The map was generated with 77 F1 progeny derived from crossing an isolate from the U.S.A. and an isolate from Syria. A total of 232 DAF (DNA Amplification Fingerprinting) primers and 37 STMS (Sequence-Tagged Microsatellite Site) primer pairs were tested for polymorphism between the parental isolates; 50 markers were mapped, 36 DAFs and 14 STMSs. These markers cover 261.4cM in ten linkage groups. Nineteen markers remained unlinked. Significant deviation from the expected 1:1 segregation ratios was observed for only two markers (Prob. of χ2 <0.05). The implications of our results on ploidy level of the asexual spores are discussed.
Note:
Related Files :
Ascochyta blight
Ascochyta rabiei
chickpea
Cicer arietinum
DAF
Didymella rabiei
Linkage map
Molecular markers
Ploidy level
STMS
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
27790
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:34
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Scientific Publication
Towards the first linkage map of the Didymella rabiei genome
30
Lichtenzveig, J., Dept. of Fld. Crops, Veg./Genet., Hebrew University of Jerusalem, Faculty of Agricultural Sciences, Rehovot 76100, Israel
Winter, P., Dept. of Fld. Crops, Veg./Genet., Hebrew University of Jerusalem, Faculty of Agricultural Sciences, Rehovot 76100, Israel
Abbo, S., Dept. of Fld. Crops, Veg./Genet., Hebrew University of Jerusalem, Faculty of Agricultural Sciences, Rehovot 76100, Israel
Shtienberg, D., Dept. of Fld. Crops, Veg./Genet., Hebrew University of Jerusalem, Faculty of Agricultural Sciences, Rehovot 76100, Israel
Kaiser, W.J., Dept. of Fld. Crops, Veg./Genet., Hebrew University of Jerusalem, Faculty of Agricultural Sciences, Rehovot 76100, Israel
Kahl, G., Dept. of Fld. Crops, Veg./Genet., Hebrew University of Jerusalem, Faculty of Agricultural Sciences, Rehovot 76100, Israel
Towards the first linkage map of the Didymella rabiei genome
A genetic map was developed for the ascomycete Didymella rabiei (Kovachevski) v. Arx (anamorph: Ascochyta rabiei Pass. Labr.), the causal agent of Ascochyta blight in chickpea (Cicer arietinum L.). The map was generated with 77 F1 progeny derived from crossing an isolate from the U.S.A. and an isolate from Syria. A total of 232 DAF (DNA Amplification Fingerprinting) primers and 37 STMS (Sequence-Tagged Microsatellite Site) primer pairs were tested for polymorphism between the parental isolates; 50 markers were mapped, 36 DAFs and 14 STMSs. These markers cover 261.4cM in ten linkage groups. Nineteen markers remained unlinked. Significant deviation from the expected 1:1 segregation ratios was observed for only two markers (Prob. of χ2 <0.05). The implications of our results on ploidy level of the asexual spores are discussed.
Scientific Publication
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