אסיף מאגר המחקר החקלאי

Gene activation by transfer of isolated mammalian chromosomes. II. Activation of simian virus 40 by transfer of fractionated chromosomes from transformed cells

Year:

1976

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Authors :

Volume :

70

Co-Authors:

Shani, M., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Aloni, Y., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Huberman, E., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Sachs, L., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel

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Abstract:

Metaphase chromosomes from an inducible SV40-transformed cell line with no detectable infectious virus or free virus DNA were fractionated into four fractions. The chromosomes were transferred into BSC-1 cells which are permissive for SV40-virus replication. The four chromosome fractions were analysed for their uptake by electron microscopy, for virus-DNA sequences by nucleic-acid hybridization, and for their activation of infectious virus. Chromatin material from all four fractions were observed in the cytoplasm of the cells after chromosome transfer. Virus-DNA sequences were detected by nucleic-acid hybridization in three of the four fractions, whereas infectious virus was activated after chromosome uptake of two of the fractions. The smallest chromosomes did not appear to contain either activatible SV40 genomes or integrated SV40-DNA sequences. It is suggested that transfer of fractionated chromosomes may also be useful for studies on the activation of other genes. © 1976.

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