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Relative infectivity of Theileria annulata (Dchunkovsky and Luhs, 1904) stabilates derived from female and male Hyalomma ecavatum (Koch, 1844) ticks
Year:
1982
Source of publication :
Veterinary Parasitology
Authors :
Samish, Michael
;
.
Volume :
10
Co-Authors:
Pipano, E., Department of Parasitology, The Kimron Veterinary Institute, Bet Dagan, Israel
Samish, M., Department of Parasitology, The Kimron Veterinary Institute, Bet Dagan, Israel
Krigel, Y., Department of Parasitology, The Kimron Veterinary Institute, Bet Dagan, Israel
Facilitators :
From page:
21
To page:
27
(
Total pages:
7
)
Abstract:
Partially engorged female and male Hyalomma excavatum ticks infected with Theileria annulata were triturated separately in Eagle's minimum essential medium complemented with fetal calf serum. Glycerol was added to the supernatant obtained after centrifugation and the material was frozen at-79°C. Infectivity of the stabilate was titrated by inoculating susceptible calves with dilutions of the material. Stabilate prepared from female ticks was considerably more infective on a per tick basis than stabilate from males. All calves inoculated with an estimated equivalent of 0.1 or more female ticks, or 3.3 or more male ticks, became infected. Time to fever, prepatent period, and mortality did not appear to be dose-dependent in the range of tick equivalents employed. © 1982.
Note:
Related Files :
Animal
cattle
Female
freezing
Growth, Development and Aging
Male
Parasitology
ticks
Show More
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More details
DOI :
10.1016/0304-4017(82)90003-6
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
27808
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:34
Scientific Publication
Relative infectivity of Theileria annulata (Dchunkovsky and Luhs, 1904) stabilates derived from female and male Hyalomma ecavatum (Koch, 1844) ticks
10
Pipano, E., Department of Parasitology, The Kimron Veterinary Institute, Bet Dagan, Israel
Samish, M., Department of Parasitology, The Kimron Veterinary Institute, Bet Dagan, Israel
Krigel, Y., Department of Parasitology, The Kimron Veterinary Institute, Bet Dagan, Israel
Relative infectivity of Theileria annulata (Dchunkovsky and Luhs, 1904) stabilates derived from female and male Hyalomma ecavatum (Koch, 1844) ticks
Partially engorged female and male Hyalomma excavatum ticks infected with Theileria annulata were triturated separately in Eagle's minimum essential medium complemented with fetal calf serum. Glycerol was added to the supernatant obtained after centrifugation and the material was frozen at-79°C. Infectivity of the stabilate was titrated by inoculating susceptible calves with dilutions of the material. Stabilate prepared from female ticks was considerably more infective on a per tick basis than stabilate from males. All calves inoculated with an estimated equivalent of 0.1 or more female ticks, or 3.3 or more male ticks, became infected. Time to fever, prepatent period, and mortality did not appear to be dose-dependent in the range of tick equivalents employed. © 1982.
Scientific Publication
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