אסיף מאגר המחקר החקלאי

Detection of potato virus Y in the aphid Myzus persicae by enzyme‐linked immunosorbent assay (ELISA)

Year:

1982

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Volume :

101

Co-Authors:

CARLEBACH, R., Virus Laboratory, Agricultural Research Organization, The Volcani Centre, Bet Dagan, Israel
RACCAH, B., Virus Laboratory, Agricultural Research Organization, The Volcani Centre, Bet Dagan, Israel
LOEBENSTEIN, G., Virus Laboratory, Agricultural Research Organization, The Volcani Centre, Bet Dagan, Israel

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Abstract:

Potato virus Y was detected by enzyme linked immunosorbent assay (ELISA) in at least 50% of groups of five Myzus persicae. The mean A405 value for groups of viruliferous aphids was 2–3 times greater than that for virus‐free ones. PVY was not detected in Aphis craccivora, A. citricola or A. gossypii, three other species which transmitted the virus to peppers, and it was detected in only a small proportion of groups of Acyrthosiphon pisum. In a series of trials, success in detection of PVY by ELISA was not correlated with the ability of other aphids from the same source plant to transmit the virus to test plants. The limitation of ELISA for quantitative assay of PVY in aphids and for epidemiological work is discussed. Copyright © 1982, Wiley Blackwell. All rights reserved

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