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Detection of potato virus Y in the aphid Myzus persicae by enzyme‐linked immunosorbent assay (ELISA)
Year:
1982
Source of publication :
Annals of Applied Biology
Authors :
Loebenstein, Gad
;
.
Raccah, Benjamin
;
.
Volume :
101
Co-Authors:
CARLEBACH, R., Virus Laboratory, Agricultural Research Organization, The Volcani Centre, Bet Dagan, Israel
RACCAH, B., Virus Laboratory, Agricultural Research Organization, The Volcani Centre, Bet Dagan, Israel
LOEBENSTEIN, G., Virus Laboratory, Agricultural Research Organization, The Volcani Centre, Bet Dagan, Israel
Facilitators :
From page:
511
To page:
516
(
Total pages:
6
)
Abstract:
Potato virus Y was detected by enzyme linked immunosorbent assay (ELISA) in at least 50% of groups of five Myzus persicae. The mean A405 value for groups of viruliferous aphids was 2–3 times greater than that for virus‐free ones. PVY was not detected in Aphis craccivora, A. citricola or A. gossypii, three other species which transmitted the virus to peppers, and it was detected in only a small proportion of groups of Acyrthosiphon pisum. In a series of trials, success in detection of PVY by ELISA was not correlated with the ability of other aphids from the same source plant to transmit the virus to test plants. The limitation of ELISA for quantitative assay of PVY in aphids and for epidemiological work is discussed. Copyright © 1982, Wiley Blackwell. All rights reserved
Note:
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More details
DOI :
10.1111/j.1744-7348.1982.tb00852.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
27818
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:34
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Scientific Publication
Detection of potato virus Y in the aphid Myzus persicae by enzyme‐linked immunosorbent assay (ELISA)
101
CARLEBACH, R., Virus Laboratory, Agricultural Research Organization, The Volcani Centre, Bet Dagan, Israel
RACCAH, B., Virus Laboratory, Agricultural Research Organization, The Volcani Centre, Bet Dagan, Israel
LOEBENSTEIN, G., Virus Laboratory, Agricultural Research Organization, The Volcani Centre, Bet Dagan, Israel
Detection of potato virus Y in the aphid Myzus persicae by enzyme‐linked immunosorbent assay (ELISA)
Potato virus Y was detected by enzyme linked immunosorbent assay (ELISA) in at least 50% of groups of five Myzus persicae. The mean A405 value for groups of viruliferous aphids was 2–3 times greater than that for virus‐free ones. PVY was not detected in Aphis craccivora, A. citricola or A. gossypii, three other species which transmitted the virus to peppers, and it was detected in only a small proportion of groups of Acyrthosiphon pisum. In a series of trials, success in detection of PVY by ELISA was not correlated with the ability of other aphids from the same source plant to transmit the virus to test plants. The limitation of ELISA for quantitative assay of PVY in aphids and for epidemiological work is discussed. Copyright © 1982, Wiley Blackwell. All rights reserved
Scientific Publication
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