אסיף מאגר המחקר החקלאי

Metabolic engineering of plant cells for biotransformation of hesperedin into neohesperidin, a substrate for production of the low-calorie sweetener and flavor enhancer NHDC

Year:

2005

Source of publication :

Authors :

Volume :

53

Co-Authors:

Frydman, A., Institute of Horticulture, Volcani Center, ARO, P.O. Box 6, Bet-Dagan 50250, Israel
Weisshaus, O., Institute of Horticulture, Volcani Center, ARO, P.O. Box 6, Bet-Dagan 50250, Israel
Huhman, D.V., Plant Biology Division, Samuel Roberts Noble Foundation, P.O. Box 2180, Ardmore, OK 73402, United States
Sumner, L.W., Plant Biology Division, Samuel Roberts Noble Foundation, P.O. Box 2180, Ardmore, OK 73402, United States
Bar-Peled, M., Department of Plant Biology, Complex Carbohydrate Research Center (CCRC), University of Georgia, 315 Riverbend Road, Athens, GA 30602-4712, United States
Lewinsohn, E., Institute of Field Crops, Newe-Ya'ar Research Center, ARO, Ramat-Yishay 30095, Israel
Fluhr, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Gressel, J., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Eyal, Y., Institute of Horticulture, Volcani Center, ARO, P.O. Box 6, Bet-Dagan 50250, Israel

Facilitators :

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Abstract:

Neohesperidin dihydrochalcone (NHDC) is a seminatural, safe, low-calorie sweetener, bitterness blocker, and flavor enhancer with unique properties and applications for the food, beverage, pharmaceutical, and animal feed industries. Current production is limited by the availability of the substrate neohesperidin, a flavonoid that accumulates to significant levels only in the inedible bitter citrus species. We propose a process to convert hesperidin, a tasteless flavonoid extracted from orange peels that are abundant byproducts of the vast orange juice industry, into neohesperidin using metabolic engineering and biotransformation via three steps: (i) extraction of hesperidin from orange peels, (ii) hydrolysis of sugar moieties, and (iii) biotransformation of hesperidin hydrolysis products into neohesperidin. We overcame the current technological bottleneck in biotransformation of hesperidin hydrolysis products into neohesperidin using metabolically engineered plant cell cultures expressing a recombinant flavanone-7-O-glucoside-2-O-rhamnosyltransferase. A small-scale production experiment established the feasibility of the proposed process. © 2005 American Chemical Society.

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