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An integrated genetic linkage map of avocado
Year:
1997
Source of publication :
Theoretical and Applied Genetics
Authors :
Lahav, Emanuel
;
.
Lavi, Uri
;
.
Mhameed, Samir
;
.
Sharon, Dror
;
.
Volume :
95
Co-Authors:
Sharon, D., Dept. Fruit Trees, Genet. and Breed., Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Cregan, P.B., USDA, ARS, Soybean and Alfalfa Res. Laboratory, Beltsville, MD 20705, United States
Mhameed, S., Dept. Fruit Trees, Genet. and Breed., Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Kusharska, M., Department of Genetics, Silesian University, Jagiallonska 28, Katovice 40032, Poland
Hillel, J., Department of Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Lahav, E., Dept. Fruit Trees, Genet. and Breed., Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Lavi, U., Dept. Fruit Trees, Genet. and Breed., Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Facilitators :
From page:
911
To page:
921
(
Total pages:
11
)
Abstract:
An avocado genomic library was screened with various microsatellite repeats. (A/T)(n) and (TC/AG)(n) sequences were found to be the most frequent repeats. One hundred and seventy-two positive clones were sequenced successfully of which 113 were found to contain simple sequence repeats (SSR). Polymerase chain reaction primers were designed to the regions flanking the SSR in 62 clones. A GenBank search of avocado DNA sequences revealed 1 sequence containing a (CT)10 repeat. A total of 92 avocado-specific SSR markers were screened for polymorphism using 50 offspring of a cross between the avocado cultivars 'Pinkerton' and 'Ettinger'. Both are standard avocado cultivars which are normally outcrossed and highly heterozygous. Fifty polymorphic SSR loci, 17 random amplified polymorphic DNA (RAPD) and 23 minisatellite DNA Fingerprint (DFP) bands were used to construct the avocado genetic map. The resulting data were analyzed with various mapping programs in order to assess which program best accommodated data from progeny of heterozygous parents. The analyses resulted in 12 linkage groups with 34 markers (25 SSRs, 3 RAPDs and 6 DFP bands) covering 352.6 cM. This initial map can serve as a basis for developing a detailed genomic map and for detection of linkage between markers and quantitative trait loci.
Note:
Related Files :
Linkage groups
Mendelian inheritance Microsatellites
Minisatellites
Simple sequence repeats
Variable number of tandem repeats
Show More
Related Content
More details
DOI :
10.1007/s001220050642
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
27865
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:34
Scientific Publication
An integrated genetic linkage map of avocado
95
Sharon, D., Dept. Fruit Trees, Genet. and Breed., Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Cregan, P.B., USDA, ARS, Soybean and Alfalfa Res. Laboratory, Beltsville, MD 20705, United States
Mhameed, S., Dept. Fruit Trees, Genet. and Breed., Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Kusharska, M., Department of Genetics, Silesian University, Jagiallonska 28, Katovice 40032, Poland
Hillel, J., Department of Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Lahav, E., Dept. Fruit Trees, Genet. and Breed., Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Lavi, U., Dept. Fruit Trees, Genet. and Breed., Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
An integrated genetic linkage map of avocado
An avocado genomic library was screened with various microsatellite repeats. (A/T)(n) and (TC/AG)(n) sequences were found to be the most frequent repeats. One hundred and seventy-two positive clones were sequenced successfully of which 113 were found to contain simple sequence repeats (SSR). Polymerase chain reaction primers were designed to the regions flanking the SSR in 62 clones. A GenBank search of avocado DNA sequences revealed 1 sequence containing a (CT)10 repeat. A total of 92 avocado-specific SSR markers were screened for polymorphism using 50 offspring of a cross between the avocado cultivars 'Pinkerton' and 'Ettinger'. Both are standard avocado cultivars which are normally outcrossed and highly heterozygous. Fifty polymorphic SSR loci, 17 random amplified polymorphic DNA (RAPD) and 23 minisatellite DNA Fingerprint (DFP) bands were used to construct the avocado genetic map. The resulting data were analyzed with various mapping programs in order to assess which program best accommodated data from progeny of heterozygous parents. The analyses resulted in 12 linkage groups with 34 markers (25 SSRs, 3 RAPDs and 6 DFP bands) covering 352.6 cM. This initial map can serve as a basis for developing a detailed genomic map and for detection of linkage between markers and quantitative trait loci.
Scientific Publication
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