אסיף מאגר המחקר החקלאי

Synergistic activity of a Bacillus thuringiensis δ-endotoxin and a bacterial endochitinase against Spodoptera littoralis larvae

Year:

1996

Source of publication :

Authors :

Volume :

62

Co-Authors:

Regev, A., Department of Botany, George S. Wise Fac. of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel
Keller, M., Department of Botany, George S. Wise Fac. of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel
Strizhov, N., Otto Warburg Ctr. Agric. Biotech., Faculty of Agriculture, Hebrew University, Rehovot 76100, Israel
Sneh, B., Department of Botany, George S. Wise Fac. of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel
Prudovsky, E., Department of Botany, George S. Wise Fac. of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel
Chet, I., Max Planck Inst. Zuchtungsforschung, Cologne 50829, Germany
Ginzberg, I., Department of Botany, George S. Wise Fac. of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel
Koncz-Kalman, Z., Otto Warburg Ctr. Agric. Biotech., Faculty of Agriculture, Hebrew University, Rehovot 76100, Israel
Koncz, C., Otto Warburg Ctr. Agric. Biotech., Faculty of Agriculture, Hebrew University, Rehovot 76100, Israel
Schell, J., Otto Warburg Ctr. Agric. Biotech., Faculty of Agriculture, Hebrew University, Rehovot 76100, Israel
Zilberstein, A., Department of Botany, George S. Wise Fac. of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel

Facilitators :

Link :

Abstract:

In an attempt to increase the insecticidal effect of the δ-endotoxin crystal protein CryIC on the relatively Cry-insensitive larvae of Spodoptera littoralis, a combination of CryIC and endochitinase was used. CryIC comprising the first 756 amino acids from Bacillus thuringiensis K26-21 and endochitinase ChiAII encoded by Serratia marcescens were separately produced in Escherichia coli carrying the genes in overexpression vectors. The endochitinase on its own, even at very low concentrations (0.1 μg/ml), perforated the larval midgut peritrophic membrane. When applied together with low concentrations of CryIC, a synergistic toxic effect was obtained. In the absence of chitinase, about 20 μg of CryIC per ml was required to obtain maximal reduction in larval weight, while only 3.0 μg of CryIC per ml caused a similar toxic effect in the presence of endochitinase. Thus, a combination of the Cry protein and an endochitinase could result in effective insect control in transgenic systems in which the Cry protein is not expressed in a crystalline form.

Note:

Related Files :

Related Content

More details

You may also be interested in