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Chronic exposure to Δ9-tetrahydrocannabinol downregulates oxytocin and oxytocin-associated neurophysin in specific brain areas
Year:
2006
Authors :
Altstein, Miriam
;
.
Volume :
31
Co-Authors:
Butovsky, E., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Juknat, A., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Elbaz, J., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Shabat-Simon, M., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Eilam, R., Department of Experimental Animals, Weizmann Institute of Science, Rehovot, Israel
Zangen, A., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Altstein, M., Department of Entomology, Volcani Center, Bet Dagan, Israel
Vogel, Z., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Facilitators :
From page:
795
To page:
804
(
Total pages:
10
)
Abstract:
Cannabinoids are widely abused drugs. Our goal was to identify genes modulated by Δ9-tetrahydrocannabinol (Δ9-THC) treatment. We found that chronic administration of Δ9-THC (1.5 mg/kg/day, i.p.; 7 days) to rats, downregulates the expression of oxytocin-neurophysin (OT-NP) mRNA and of OT and oxytocin-associated NP (NP OT) immunoreactivity in nucleus accumbens (NAc) and ventral tegmental area (VTA), brain areas involved in reward and addiction. Real-time PCR revealed a 60% and 53% reduction of OT-NP mRNA in NAc and VTA, respectively, under chronic treatment, while no changes were observed in NAc after 24 h. Immunohistochemistry showed a large decrease in number of OT and NP OT-stained fibers in NAc (by 59% and 52%, respectively) and VTA (by 50% and 56%, respectively). No changes in cell staining were observed in the paraventricular nucleus and supraoptic nucleus. As OT is known to inhibit development of drug tolerance and attenuate withdrawal symptoms, we suggest that OT downregulation could play a role during the establishment of the chronic effects of Δ9-THC. © 2006 Elsevier Inc. All rights reserved.
Note:
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drug effect
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metabolism
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More details
DOI :
10.1016/j.mcn.2006.01.008
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
27928
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:35
Scientific Publication
Chronic exposure to Δ9-tetrahydrocannabinol downregulates oxytocin and oxytocin-associated neurophysin in specific brain areas
31
Butovsky, E., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Juknat, A., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Elbaz, J., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Shabat-Simon, M., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Eilam, R., Department of Experimental Animals, Weizmann Institute of Science, Rehovot, Israel
Zangen, A., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Altstein, M., Department of Entomology, Volcani Center, Bet Dagan, Israel
Vogel, Z., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Chronic exposure to Δ9-tetrahydrocannabinol downregulates oxytocin and oxytocin-associated neurophysin in specific brain areas
Cannabinoids are widely abused drugs. Our goal was to identify genes modulated by Δ9-tetrahydrocannabinol (Δ9-THC) treatment. We found that chronic administration of Δ9-THC (1.5 mg/kg/day, i.p.; 7 days) to rats, downregulates the expression of oxytocin-neurophysin (OT-NP) mRNA and of OT and oxytocin-associated NP (NP OT) immunoreactivity in nucleus accumbens (NAc) and ventral tegmental area (VTA), brain areas involved in reward and addiction. Real-time PCR revealed a 60% and 53% reduction of OT-NP mRNA in NAc and VTA, respectively, under chronic treatment, while no changes were observed in NAc after 24 h. Immunohistochemistry showed a large decrease in number of OT and NP OT-stained fibers in NAc (by 59% and 52%, respectively) and VTA (by 50% and 56%, respectively). No changes in cell staining were observed in the paraventricular nucleus and supraoptic nucleus. As OT is known to inhibit development of drug tolerance and attenuate withdrawal symptoms, we suggest that OT downregulation could play a role during the establishment of the chronic effects of Δ9-THC. © 2006 Elsevier Inc. All rights reserved.
Scientific Publication
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