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Translational competition between related virus RNA species in cell-free systems
Year:
1982
Source of publication :
Journal of General Virology
Authors :
Bar-Joseph, Moshe
;
.
Salomon, Raffi
;
.
Volume :
62
Co-Authors:
Salomon, R., Virus Lab., Agric. Res. Organ., Volcani Cent., Bet Dagan 50-250, Israel
Bar-Joseph, M., Virus Lab., Agric. Res. Organ., Volcani Cent., Bet Dagan 50-250, Israel
Facilitators :
From page:
343
To page:
347
(
Total pages:
5
)
Abstract:
The competition between the RNAs from two tobacco mosaic virus (TMV) strains for translation in rabbit reticulocyte lysate and wheat germ cell-free systems was investigated. The virus translational products were distinguished by the size difference of the I 2 polypeptides. The RNAs of the two strains were translated simultaneously if added to the translation system together at the beginning of the reaction. Each of the TMV RNAs could inhibit translation of the other when the reaction was initiated by the RNA of one strain and that of the other strain was added after active translation had commenced. In contrast, translation of alfalfa mosaic virus (AMV) RNA was not appreciably inhibited when its RNA was added after the start of translation of TMV RNA; the addition of TMV RNA to an AMV RNA-initiated reaction also resulted in independent synthesis of the products of both virus RNAs.
Note:
Related Files :
Cell-Free System
Kinetics
plant
reticulocyte count
RNA, Messenger
Tobacco mosaic virus
Translation, Genetic
virus replication
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Related Content
More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
27974
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:35
Scientific Publication
Translational competition between related virus RNA species in cell-free systems
62
Salomon, R., Virus Lab., Agric. Res. Organ., Volcani Cent., Bet Dagan 50-250, Israel
Bar-Joseph, M., Virus Lab., Agric. Res. Organ., Volcani Cent., Bet Dagan 50-250, Israel
Translational competition between related virus RNA species in cell-free systems
The competition between the RNAs from two tobacco mosaic virus (TMV) strains for translation in rabbit reticulocyte lysate and wheat germ cell-free systems was investigated. The virus translational products were distinguished by the size difference of the I 2 polypeptides. The RNAs of the two strains were translated simultaneously if added to the translation system together at the beginning of the reaction. Each of the TMV RNAs could inhibit translation of the other when the reaction was initiated by the RNA of one strain and that of the other strain was added after active translation had commenced. In contrast, translation of alfalfa mosaic virus (AMV) RNA was not appreciably inhibited when its RNA was added after the start of translation of TMV RNA; the addition of TMV RNA to an AMV RNA-initiated reaction also resulted in independent synthesis of the products of both virus RNAs.
Scientific Publication
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