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Lipkin, E., Department of Genetics, Hebrew University of Jerusalem, Jerusalem, Israel
Strillacci, M.G., Universita degli Studi di Milano (UNIMI), Milan, Italy
Eitam, H., Department of Ruminant Sciences, Agricultural Research Organization (ARO), Bet-Dagan, Israel
Yishay, M., Department of Ruminant Sciences, Agricultural Research Organization (ARO), Bet-Dagan, Israel
Schiavini, F., Universita degli Studi di Milano (UNIMI), Milan, Italy
Soller, M., Department of Genetics, Hebrew University of Jerusalem, Jerusalem, Israel
Bagnato, A., Universita degli Studi di Milano (UNIMI), Milan, Italy
Shabtay, A., Department of Ruminant Sciences, Agricultural Research Organization (ARO), Bet-Dagan, Israel
Bovine respiratory disease (BRD) is the leading cause of morbidity and mortality in feedlot cattle, caused by multiple pathogens that become more virulent in response to stress. As clinical signs often go undetected and various preventive strategies failed, identification of genes affecting BRD is essential for selection for resistance. Selective DNA pooling (SDP) was applied in a genome wide association study (GWAS) to map BRD QTLs in Israeli Holstein male calves. Kosher scoring of lung adhesions was used to allocate 122 and 62 animals to High (Glatt Kosher) and Low (Non-Kosher) resistant groups, respectively. Genotyping was performed using the Illumina BovineHD BeadChip according to the Infinium protocol. Moving average of -logP was used to map QTLs and Log drop was used to define their boundaries (QTLRs). The combined procedure was efficient for high resolution mapping. Nineteen QTLRs distributed over 13 autosomes were found, some overlapping previous studies. The QTLRs contain polymorphic functional and expression candidate genes to affect kosher status, with putative immunological and wound healing activities. Kosher phenotyping was shown to be a reliable means to map QTLs affecting BRD morbidity. © 2016 Lipkin et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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The use of Kosher phenotyping for mapping QTL affecting susceptibility to Bovine respiratory disease
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Lipkin, E., Department of Genetics, Hebrew University of Jerusalem, Jerusalem, Israel
Strillacci, M.G., Universita degli Studi di Milano (UNIMI), Milan, Italy
Eitam, H., Department of Ruminant Sciences, Agricultural Research Organization (ARO), Bet-Dagan, Israel
Yishay, M., Department of Ruminant Sciences, Agricultural Research Organization (ARO), Bet-Dagan, Israel
Schiavini, F., Universita degli Studi di Milano (UNIMI), Milan, Italy
Soller, M., Department of Genetics, Hebrew University of Jerusalem, Jerusalem, Israel
Bagnato, A., Universita degli Studi di Milano (UNIMI), Milan, Italy
Shabtay, A., Department of Ruminant Sciences, Agricultural Research Organization (ARO), Bet-Dagan, Israel
The use of Kosher phenotyping for mapping QTL affecting susceptibility to Bovine respiratory disease
Bovine respiratory disease (BRD) is the leading cause of morbidity and mortality in feedlot cattle, caused by multiple pathogens that become more virulent in response to stress. As clinical signs often go undetected and various preventive strategies failed, identification of genes affecting BRD is essential for selection for resistance. Selective DNA pooling (SDP) was applied in a genome wide association study (GWAS) to map BRD QTLs in Israeli Holstein male calves. Kosher scoring of lung adhesions was used to allocate 122 and 62 animals to High (Glatt Kosher) and Low (Non-Kosher) resistant groups, respectively. Genotyping was performed using the Illumina BovineHD BeadChip according to the Infinium protocol. Moving average of -logP was used to map QTLs and Log drop was used to define their boundaries (QTLRs). The combined procedure was efficient for high resolution mapping. Nineteen QTLRs distributed over 13 autosomes were found, some overlapping previous studies. The QTLRs contain polymorphic functional and expression candidate genes to affect kosher status, with putative immunological and wound healing activities. Kosher phenotyping was shown to be a reliable means to map QTLs affecting BRD morbidity. © 2016 Lipkin et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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