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Antiviral activity of natural and recombinant human leukocyte interferons in tobacco protoplasts
Year:
1985
Source of publication :
Virology
Authors :
Gera, Abdullah
;
.
Volume :
140
Co-Authors:
Rosenberg, N., Virus Laboratory, The Hebrew University, Faculty of Agriculture, Rehovot, 76100, Israel
Reichman, M., Virus Laboratory, The Hebrew University, Faculty of Agriculture, Rehovot, 76100, Israel
Gera, A., Virus Laboratory, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Sela, I., Virus Laboratory, The Hebrew University, Faculty of Agriculture, Rehovot, 76100, Israel
Facilitators :
From page:
173
To page:
178
(
Total pages:
6
)
Abstract:
Several purified species of human leukocyte interferon, including recombinant interferons, inhibit the multiplication of tobacco mosaic virus (TMV) in tobacco protoplasts derived from various cultivars. Viral RNA accumulation was determined by dot-blot hybridization to specific cDNA probes, and virus antigen was determined serologically. Interferon apparently inhibited both TMV-RNA replication and its expression into coat protein. However, these effects were of limited duration. Maximum effect was obtained when interferon was applied to the cells either prior to inoculation or within the first hour after inoculation. Antibodies to interferon abolished its antiviral activity in protoplasts. Tobacco protoplasts were about 1000 times more responsive to interferon than the reference animal viral-cell system and showed an "antiviral state" at a ratio of 1 molecule of interferon per cell. © 1985.
Note:
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More details
DOI :
10.1016/0042-6822(85)90456-8
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28003
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:35
You may also be interested in
Scientific Publication
Antiviral activity of natural and recombinant human leukocyte interferons in tobacco protoplasts
140
Rosenberg, N., Virus Laboratory, The Hebrew University, Faculty of Agriculture, Rehovot, 76100, Israel
Reichman, M., Virus Laboratory, The Hebrew University, Faculty of Agriculture, Rehovot, 76100, Israel
Gera, A., Virus Laboratory, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Sela, I., Virus Laboratory, The Hebrew University, Faculty of Agriculture, Rehovot, 76100, Israel
Antiviral activity of natural and recombinant human leukocyte interferons in tobacco protoplasts
Several purified species of human leukocyte interferon, including recombinant interferons, inhibit the multiplication of tobacco mosaic virus (TMV) in tobacco protoplasts derived from various cultivars. Viral RNA accumulation was determined by dot-blot hybridization to specific cDNA probes, and virus antigen was determined serologically. Interferon apparently inhibited both TMV-RNA replication and its expression into coat protein. However, these effects were of limited duration. Maximum effect was obtained when interferon was applied to the cells either prior to inoculation or within the first hour after inoculation. Antibodies to interferon abolished its antiviral activity in protoplasts. Tobacco protoplasts were about 1000 times more responsive to interferon than the reference animal viral-cell system and showed an "antiviral state" at a ratio of 1 molecule of interferon per cell. © 1985.
Scientific Publication
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