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British Journal of Nutrition
sklan, D., The Volcani Institute of Agricultural Researh, The Faculty of Agriculture, The Hebrew University of Jerusalem, Bet Dagan, Rehovoi, Israel
budowski, P., The Volcani Institute of Agricultural Researh, The Faculty of Agriculture, The Hebrew University of Jerusalem, Bet Dagan, Rehovoi, Israel
volcani, R., The Volcani Institute of Agricultural Researh, The Faculty of Agriculture, The Hebrew University of Jerusalem, Bet Dagan, Rehovoi, Israel
Rumen liquor from calves 3–5 weeks old, and a supernatant fraction prepared by centrifugation from rumen fluid of older calves showed an increase in octadecadienoic acid during aerobic incubation, but not during incubation under strictly anaerobic conditions. 2. The lipoxygenase test revealed the presence of the cw, m-i, 4-pentadiene system in the product formed on incubation, whereas oxidative degradation showed it to belong to the w6 family. The results point to linoleic acid as the principal product formed. 3. Incubations in the presence of [i-14C]oleic acid resulted in the incorporation of 4-7% of the label into the octadecadienoic acid fraction, whereas similar tests with labelled stearic acid caused about 1 % incorporation. 4. These results, in conjunction with the observation that the increase in 18:2 is always accompanied by a decrease in 18:0 + 18:1 of approximately equal magnitude, indicate that linoleate is being formed by aerobic desaturation of more saturated fatty acids. © 1972, The Nutrition Society. All rights reserved.
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Synthesis in vitro of linoleic acid by rumen liquor of calves
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sklan, D., The Volcani Institute of Agricultural Researh, The Faculty of Agriculture, The Hebrew University of Jerusalem, Bet Dagan, Rehovoi, Israel
budowski, P., The Volcani Institute of Agricultural Researh, The Faculty of Agriculture, The Hebrew University of Jerusalem, Bet Dagan, Rehovoi, Israel
volcani, R., The Volcani Institute of Agricultural Researh, The Faculty of Agriculture, The Hebrew University of Jerusalem, Bet Dagan, Rehovoi, Israel
Synthesis in vitro of linoleic acid by rumen liquor of calves
Rumen liquor from calves 3–5 weeks old, and a supernatant fraction prepared by centrifugation from rumen fluid of older calves showed an increase in octadecadienoic acid during aerobic incubation, but not during incubation under strictly anaerobic conditions. 2. The lipoxygenase test revealed the presence of the cw, m-i, 4-pentadiene system in the product formed on incubation, whereas oxidative degradation showed it to belong to the w6 family. The results point to linoleic acid as the principal product formed. 3. Incubations in the presence of [i-14C]oleic acid resulted in the incorporation of 4-7% of the label into the octadecadienoic acid fraction, whereas similar tests with labelled stearic acid caused about 1 % incorporation. 4. These results, in conjunction with the observation that the increase in 18:2 is always accompanied by a decrease in 18:0 + 18:1 of approximately equal magnitude, indicate that linoleate is being formed by aerobic desaturation of more saturated fatty acids. © 1972, The Nutrition Society. All rights reserved.
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