Analysis of Ly-1+ B-Cell Populations and IgH Rearrangements in “Normal” Spleens and in Lymphomas of AKR/J and AKR Fv-1b Mice

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Analysis of Ly-1+ B-Cell Populations and IgH Rearrangements in “Normal” Spleens and in Lymphomas of AKR/J and AKR Fv-1b Mice

Year: 

1993

Source of publication :

Cancer Research

Authors :

Rosner, Arie

.

Volume :

Co-Authors: 

Rosner, A., Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel
Peled, A., Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel
Haran-Ghera, N., Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel
Canaani, E., Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel

From page: 

2147

To page: 

2153

(

Total pages: 

7

)

Link :

Analysis of Ly-1+ B-Cell Populations and IgH Rearrangements in “Normal” Spleens and in Lymphomas of AKR/J and AKR Fv-1b Mice

Abstract: 

AKR mice are highly susceptible to development of spontaneous T-cell lymphoma. Thymus removal at the age of 1-3 months greatly reduces T-cell lymphoma. Lymphomas that have the characteristics of T- and/or B-cells occur sporadically in peripheral lymphoid tissues of old thymec-tomized AKR/J mice. These thymectomized mice were shown to carry dormant potential lymphoma cells. Transplantation of lymphoid cells from 8-12-month-old AKR/J mice, thymectomized at the age of 6 to 8 weeks, into intact or thymectomized young recipients yielded 80-100% Ly-1+ pre-B or B-cell lymphomas. In the AKR-Fv-lb congenic strain the Fv-r allele of AKR/J mice was substituted with the Fv-lb allele, thereby limiting viral replication and spread of the endogenous N-tropic murine leukemia virus. As a result of this restriction in virus spread, AKR-Fv-lb mice develop a low spontaneous incidence (7%) of T-cell lymphomas and about 28% of Ly-1 B-cell lymphomas at old age. In spleens of 15-18-month-old thymectomized AKR/J mice and intact AKR-Fv-lb mice, 30-60% of the B-cells were of the Ly-1+ B type. Analysis of the IgH locus in these normal old spleens and Ly-1 B lymphomas indicated mono- or oligoclonality. One particular IgH rearrangement was identified in many individual old spleens and tumors. A second specific IgH rearrangement was found in some tumors. Possible mechanisms involved in the expansion of Ly-1+ clones and their progression into tumors are discussed. © 1993, American Association for Cancer Research. All rights reserved.