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Partial purification and characterization of locust allatotropin I
Year:
1987
Authors :
Rafaeli, Ada
;
.
Volume :
4
Co-Authors:
Gadot, M., Department of Entomology, The Hebrew University, Rehovot, Israel
Rafaeli, A., Department of Entomology, The Hebrew University, Rehovot, Israel
Applebaum, S.W., Department of Entomology, The Hebrew University, Rehovot, Israel
Facilitators :
From page:
213
To page:
223
(
Total pages:
11
)
Abstract:
Methanol extracts of locust brains, corpora cardiaca (CC), and suboesophageal ganglia (SOG) were separated by gradient and/or isocratic reverse‐phase high‐performance liquid chromatography (HPLC) and allatotropic activity monitored in the eluted fractions. A major peak of activity, separated by isocratic separation with 12% 2‐propanol, designated allatotropin I, exhibited identical retention times in the three tissue extracts. Doseresponse curves of allatotropin I indicate similar content in brain and CC‐equivalents, whereas optic lobes, similarly separated by isocratic HPLC, contain only one‐tenth of this amount of allatotropin. Allatotropin I is resistant to boiling and is susceptible to tryptic and chymotryptic digestion. Methanol extracts of thoracic muscle, Malpighian tubules, fat body or ovaries, similarly prepared and boiled, did not exhibit allatotropic activity at high doses of tissue equivalents. Copyright © 1987 Wiley‐Liss, Inc.
Note:
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Locusta migratoria
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More details
DOI :
10.1002/arch.940040307
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28171
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:37
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Scientific Publication
Partial purification and characterization of locust allatotropin I
4
Gadot, M., Department of Entomology, The Hebrew University, Rehovot, Israel
Rafaeli, A., Department of Entomology, The Hebrew University, Rehovot, Israel
Applebaum, S.W., Department of Entomology, The Hebrew University, Rehovot, Israel
Partial purification and characterization of locust allatotropin I
Methanol extracts of locust brains, corpora cardiaca (CC), and suboesophageal ganglia (SOG) were separated by gradient and/or isocratic reverse‐phase high‐performance liquid chromatography (HPLC) and allatotropic activity monitored in the eluted fractions. A major peak of activity, separated by isocratic separation with 12% 2‐propanol, designated allatotropin I, exhibited identical retention times in the three tissue extracts. Doseresponse curves of allatotropin I indicate similar content in brain and CC‐equivalents, whereas optic lobes, similarly separated by isocratic HPLC, contain only one‐tenth of this amount of allatotropin. Allatotropin I is resistant to boiling and is susceptible to tryptic and chymotryptic digestion. Methanol extracts of thoracic muscle, Malpighian tubules, fat body or ovaries, similarly prepared and boiled, did not exhibit allatotropic activity at high doses of tissue equivalents. Copyright © 1987 Wiley‐Liss, Inc.
Scientific Publication
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