אסיף מאגר המחקר החקלאי

Partial purification and characterization of locust allatotropin I

Year:

1987

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Volume :

4

Co-Authors:

Gadot, M., Department of Entomology, The Hebrew University, Rehovot, Israel
Rafaeli, A., Department of Entomology, The Hebrew University, Rehovot, Israel
Applebaum, S.W., Department of Entomology, The Hebrew University, Rehovot, Israel

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Abstract:

Methanol extracts of locust brains, corpora cardiaca (CC), and suboesophageal ganglia (SOG) were separated by gradient and/or isocratic reverse‐phase high‐performance liquid chromatography (HPLC) and allatotropic activity monitored in the eluted fractions. A major peak of activity, separated by isocratic separation with 12% 2‐propanol, designated allatotropin I, exhibited identical retention times in the three tissue extracts. Doseresponse curves of allatotropin I indicate similar content in brain and CC‐equivalents, whereas optic lobes, similarly separated by isocratic HPLC, contain only one‐tenth of this amount of allatotropin. Allatotropin I is resistant to boiling and is susceptible to tryptic and chymotryptic digestion. Methanol extracts of thoracic muscle, Malpighian tubules, fat body or ovaries, similarly prepared and boiled, did not exhibit allatotropic activity at high doses of tissue equivalents. Copyright © 1987 Wiley‐Liss, Inc.

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