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A model for in vitro proliferation of undifferentiated bovine mammary epithelial cells
Year:
1986
Source of publication :
Cell Biology International Reports
Authors :
Shamay, Avi
;
.
Volume :
10
Co-Authors:
Shamay, A., Department of Agricultural Biochemistry, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.Box 12, Rehovot, 76100, Israel
Gertler, A., Department of Agricultural Biochemistry, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.Box 12, Rehovot, 76100, Israel
Facilitators :
From page:
923
To page:
929
(
Total pages:
7
)
Abstract:
A method for culturing bovine undifferentiated mammary epithelial cells embedded in collagen gels is described. Cell growth was quantitated by incorporation of tritiated thymidine. Maximal rate of incorporation was achieved in media supplemented with sera, however considerable growth was observed in serum-free medium supplemented with insulin, transferrin and selenium. The growth promoting effect of insulin was maximally expressed only at high nonphysiological concentrations. © 1986.
Note:
Related Files :
Animal
biosynthesis
Blood
cattle
Cell Division
DNA
drug effect
epithelium cell
Female
Selenium
transferrin
Show More
Related Content
More details
DOI :
10.1016/0309-1651(86)90112-8
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28187
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:37
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A model for in vitro proliferation of undifferentiated bovine mammary epithelial cells
10
Shamay, A., Department of Agricultural Biochemistry, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.Box 12, Rehovot, 76100, Israel
Gertler, A., Department of Agricultural Biochemistry, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.Box 12, Rehovot, 76100, Israel
A model for in vitro proliferation of undifferentiated bovine mammary epithelial cells
A method for culturing bovine undifferentiated mammary epithelial cells embedded in collagen gels is described. Cell growth was quantitated by incorporation of tritiated thymidine. Maximal rate of incorporation was achieved in media supplemented with sera, however considerable growth was observed in serum-free medium supplemented with insulin, transferrin and selenium. The growth promoting effect of insulin was maximally expressed only at high nonphysiological concentrations. © 1986.
Scientific Publication
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