Co-Authors:
Abu-Abied, M., Institute of Plant Sciences, Volcani Center, ARO, PO Box 6, Bet-Dagan, Israel
Rogovoy, O., Institute of Plant Sciences, Volcani Center, ARO, PO Box 6, Bet-Dagan, Israel
Mordehaev, I., Institute of Plant Sciences, Volcani Center, ARO, PO Box 6, Bet-Dagan, Israel
Grumberg, M., Institute of Plant Sciences, Volcani Center, ARO, PO Box 6, Bet-Dagan, Israel
Elbaum, R., Smith Institute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot, Israel
Wasteneys, G.O., Department of Botany, University of British Columbia, 6270 University Boulevard, Vancouver, BC, Canada
Sadot, E., Institute of Plant Sciences, Volcani Center, ARO, PO Box 6, Bet-Dagan, Israel
Abstract:
Induction of adventitious roots (ARs) in recalcitrant plants often culminates in cell division and callus formation rather than root differentiation. Evidence is provided here to suggest that microtubules (MTs) play a role in the shift from cell division to cell differentiation during AR induction. First, it was found that fewer ARs form in the temperature-sensitive mutant mor1-1, in which the MT-associated protein MOR1 is mutated, and in bot1-1, in which the MT-severing protein katanin is mutated. In the two latter mutants, MT dynamics and form are perturbed. By contrast, the number of ARs increased in RIC1-OX3 plants, in which MT bundling is enhanced and katanin is activated. In addition, any1 plants in which cell walls are perturbed made more ARs than wild-type plants. MT perturbations during AR induction in mor1-1 or in wild-type hypocotyls treated with oryzalin led to the formation of amorphous clusters of cells reminiscent of callus. In these cells a specific pattern of polarized light retardation by the cell walls was lost. PIN1 polarization and auxin maxima were hampered and differentiation of the epidermis was inhibited. It is concluded that a fine-tuned crosstalk between MTs, cell walls, and auxin transport is required for proper AR induction. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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