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Heterogeneity of the genes coding for 5 S RNA in three related strains of the genus Bacillus
Year:
1977
Source of publication :
MGG Molecular & General Genetics
Authors :
Rosner, Arie
;
.
Volume :
156
Co-Authors:
Raué, H.A., Biochemisch Laboratorium, Vrije Universiteit, De Boelelaan 1085, Amsterdam, Netherlands
Rosner, A., Biochemisch Laboratorium, Vrije Universiteit, De Boelelaan 1085, Amsterdam, Netherlands
Planta, R.J., Biochemisch Laboratorium, Vrije Universiteit, De Boelelaan 1085, Amsterdam, Netherlands
Facilitators :
From page:
185
To page:
193
(
Total pages:
9
)
Abstract:
Three related strains of the genus Bacillus, viz. B. licheniformis, B. subtilis and Bacillus Q were all found to contain a minor species of 5 S RNA in an amount indicating that it is transcribed from only one of the multiple 5 S RNA cistrons known to be present in these strains. The major and minor types of 5 S RNA from each of the three strains could be separated from each other by polyacrylamide gel electrophoresis in the presence of urea. The complete nucleotide sequences of the minor B. subtilis and Bacillus Q 5 S RNAs have been determined. Comparison of these sequences to the previously determined sequence of the minor 5 S RNA from B. licheniformis (Raué et al., 1976) showed the three minor types of 5 S RNA to be identical except for the residues at positions 79, 85 and 95. Moreover, seven out of the eight sequence differences between the major and the minor 5 S RNA are the same in all three strains. Thus, the single cistron coding for minor 5 S RNA has been strongly conserved in all three strains, which may indicate a biological significance for the minor 5 S RNA species. © 1977 Springer-Verlag.
Note:
Related Files :
Bacillus
Base Sequence
Electrophoresis, Polyacrylamide Gel
Gene
Genes
microorganism
Variation (Genetics)
Show More
Related Content
More details
DOI :
10.1007/BF00283491
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28255
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:37
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Scientific Publication
Heterogeneity of the genes coding for 5 S RNA in three related strains of the genus Bacillus
156
Raué, H.A., Biochemisch Laboratorium, Vrije Universiteit, De Boelelaan 1085, Amsterdam, Netherlands
Rosner, A., Biochemisch Laboratorium, Vrije Universiteit, De Boelelaan 1085, Amsterdam, Netherlands
Planta, R.J., Biochemisch Laboratorium, Vrije Universiteit, De Boelelaan 1085, Amsterdam, Netherlands
Heterogeneity of the genes coding for 5 S RNA in three related strains of the genus Bacillus
Three related strains of the genus Bacillus, viz. B. licheniformis, B. subtilis and Bacillus Q were all found to contain a minor species of 5 S RNA in an amount indicating that it is transcribed from only one of the multiple 5 S RNA cistrons known to be present in these strains. The major and minor types of 5 S RNA from each of the three strains could be separated from each other by polyacrylamide gel electrophoresis in the presence of urea. The complete nucleotide sequences of the minor B. subtilis and Bacillus Q 5 S RNAs have been determined. Comparison of these sequences to the previously determined sequence of the minor 5 S RNA from B. licheniformis (Raué et al., 1976) showed the three minor types of 5 S RNA to be identical except for the residues at positions 79, 85 and 95. Moreover, seven out of the eight sequence differences between the major and the minor 5 S RNA are the same in all three strains. Thus, the single cistron coding for minor 5 S RNA has been strongly conserved in all three strains, which may indicate a biological significance for the minor 5 S RNA species. © 1977 Springer-Verlag.
Scientific Publication
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