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Phenotypic expression of marrow cells when grown on various substrata
Year:
1996
Source of publication :
Journal of Cellular Biochemistry
Authors :
Shamay, Avi
;
.
Volume :
61
Co-Authors:
Fried, A., Division of Orthopaedics, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 64239, Israel
Shamay, A., Division of Orthopaedics, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 64239, Israel
Wientroub, S., Division of Orthopaedics, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 64239, Israel, Department of Pediatric Orthopaedics, Dana Children's Hospital, Tel-Aviv Sourasky Medical Center, Tel-Aviv 64239, Israel
Benayahu, D., Department of Cell Biology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 64239, Israel, Dept. of Cell Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 66978, Israel
Facilitators :
From page:
246
To page:
254
(
Total pages:
9
)
Abstract:
Our aim was to study the role of various extracellular matrices (ECM) on growth and differentiation of marrow stromal cells in vitro. Morphology changes, gene expression, and enzymatic activities were monitored in stromal osteoblastic MBA-15 and adipocytic 14F1.1 cells. These stromal cells were plated on dishes precoated with different substrata, such as matrigel (basement membrane), collagen type I, and endothelial ECM, and compared with cells plated on protein-free dishes. Striking morphological differences were observed when the cells grew on these different substrata. Changes in cell shape and growth also led to differential mRNA expression and enzymatic activities. When MBA-15 cells were plated on collagen, there was a decrease in mRNA for alkaline phosphatase (ALK-P), osteopontin (OP), and osteonectin (ON), and an increase in mRNA for procollagen (I). A differential effect was noted on 14F1.1 cells, the mRNA for ALK-P increased, the expressions of OP and ON lowered, and no expression for procollagen (1) was monitored. MBA-15 cells cultured on matrigel had decreased mRNA for ALK-P and OP, while they had increased ON mRNA expression and remained unchanged for procollagen I. No change in mRNA expression by 14F1.1 cells was monitored when cultured on matrigel. Functional enzymatic activities of ALK-P markedly decreased in MBA- 15 cells cultured on various substrata, and increased or were unchanged in 14F1.1 cells. An additional enzyme, neutral endopeptidase (CD10/NEP), altered differentially in both cell types; this enzymatic activity increased or was unchanged when cells were cultured on these matrices. The results indicate a specific role for different ECM on various stromal cell types and their function.
Note:
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More details
DOI :
10.1002/(SICI)1097-4644(19960501)61:2<246::AID-JCB8>3.0.CO;2-U
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28269
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:37
Scientific Publication
Phenotypic expression of marrow cells when grown on various substrata
61
Fried, A., Division of Orthopaedics, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 64239, Israel
Shamay, A., Division of Orthopaedics, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 64239, Israel
Wientroub, S., Division of Orthopaedics, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 64239, Israel, Department of Pediatric Orthopaedics, Dana Children's Hospital, Tel-Aviv Sourasky Medical Center, Tel-Aviv 64239, Israel
Benayahu, D., Department of Cell Biology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 64239, Israel, Dept. of Cell Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 66978, Israel
Phenotypic expression of marrow cells when grown on various substrata
Our aim was to study the role of various extracellular matrices (ECM) on growth and differentiation of marrow stromal cells in vitro. Morphology changes, gene expression, and enzymatic activities were monitored in stromal osteoblastic MBA-15 and adipocytic 14F1.1 cells. These stromal cells were plated on dishes precoated with different substrata, such as matrigel (basement membrane), collagen type I, and endothelial ECM, and compared with cells plated on protein-free dishes. Striking morphological differences were observed when the cells grew on these different substrata. Changes in cell shape and growth also led to differential mRNA expression and enzymatic activities. When MBA-15 cells were plated on collagen, there was a decrease in mRNA for alkaline phosphatase (ALK-P), osteopontin (OP), and osteonectin (ON), and an increase in mRNA for procollagen (I). A differential effect was noted on 14F1.1 cells, the mRNA for ALK-P increased, the expressions of OP and ON lowered, and no expression for procollagen (1) was monitored. MBA-15 cells cultured on matrigel had decreased mRNA for ALK-P and OP, while they had increased ON mRNA expression and remained unchanged for procollagen I. No change in mRNA expression by 14F1.1 cells was monitored when cultured on matrigel. Functional enzymatic activities of ALK-P markedly decreased in MBA- 15 cells cultured on various substrata, and increased or were unchanged in 14F1.1 cells. An additional enzyme, neutral endopeptidase (CD10/NEP), altered differentially in both cell types; this enzymatic activity increased or was unchanged when cells were cultured on these matrices. The results indicate a specific role for different ECM on various stromal cell types and their function.
Scientific Publication
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