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Arabidopsis fructokinases are important for seed oil accumulation and vascular development
Year:
2017
Source of publication :
Frontiers in Plant Science
Authors :
Bogol, Vlada
;
.
Stein, Ofer
;
.
Volume :
7
Co-Authors:
Stein, O., Volcani Center, Institute of Plant Sciences, Agricultural Research Organization, Bet Dagan, Israel, Robert H. Smith Faculty of Agriculture, Institute of Plant Sciences and Genetics in Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Avin-Wittenberg, T., Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany, Department of Plant and Environmental Sciences, Hebrew University of Jerusalem, Givat Ram, Jerusalem, Israel
Krahnert, I., Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany
Zemach, H., Volcani Center, Institute of Plant Sciences, Agricultural Research Organization, Bet Dagan, Israel
Bogol, V., Volcani Center, Institute of Plant Sciences, Agricultural Research Organization, Bet Dagan, Israel
Daron, O., Department of Life Sciences, Ben-Gurion University, Beer-Sheva, Israel
Aloni, R., Department of Plant Sciences, Tel Aviv University, Tel Aviv, Israel
Fernie, A.R., Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany
Granot, D., Volcani Center, Institute of Plant Sciences, Agricultural Research Organization, Bet Dagan, Israel
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
Sucrose (a disaccharide made of glucose and fructose) is the primary carbon source transported to sink organs in many plants. Since fructose accounts for half of the hexoses used for metabolism in sink tissues, plant fructokinases (FRKs), the main fructose-phosphorylating enzymes, are likely to play a central role in plant development. However, to date, their specific functions have been the subject of only limited study. The Arabidopsis genome contains seven genes encoding six cytosolic FRKs and a single plastidic FRK. T-DNA knockout mutants for five of the seven FRKs were identified and used in this study. Single knockouts of the FRK mutants did not exhibit any unusual phenotype. Double-mutants of AtFRK6 (plastidic) and AtFRK7 showed normal growth in soil, but yielded dark, distorted seeds. The seed distortion could be complemented by expression of the well-characterized tomato SlFRK1, confirming that a lack of FRK activity was the primary cause of the seed phenotype. Seeds of the double-mutant germinated, but failed to establish on 1/2 MS plates. Seed establishment was made possible by the addition of glucose or sucrose, indicating reduced seed storage reserves. Metabolic profiling of the double-mutant seeds revealed decreased TCA cycle metabolites and reduced fatty acid metabolism. Examination of the mutant embryo cells revealed smaller oil bodies, the primary storage reserve in Arabidopsis seeds. Quadruple and penta FRK mutants showed growth inhibition and leaf wilting. Anatomical analysis revealed smaller trachea elements and smaller xylem area, accompanied by necrosis around the cambium and the phloem. These results demonstrate overlapping and complementary roles of the plastidic AtFRK6 and the cytosolic AtFRK7 in seed storage accumulation, and the importance of AtFRKs for vascular development. © 2017 Stein, Avin-Wittenberg, Krahnert, Zemach, Bogol, Daron, Aloni, Fernie and Granot.
Note:
Related Files :
carbon metabolism
fatty acid
Fructokinase
Seed oil
xylem
Show More
Related Content
More details
DOI :
10.3389/fpls.2016.02047
Article number:
2047
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28313
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:38
Scientific Publication
Arabidopsis fructokinases are important for seed oil accumulation and vascular development
7
Stein, O., Volcani Center, Institute of Plant Sciences, Agricultural Research Organization, Bet Dagan, Israel, Robert H. Smith Faculty of Agriculture, Institute of Plant Sciences and Genetics in Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Avin-Wittenberg, T., Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany, Department of Plant and Environmental Sciences, Hebrew University of Jerusalem, Givat Ram, Jerusalem, Israel
Krahnert, I., Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany
Zemach, H., Volcani Center, Institute of Plant Sciences, Agricultural Research Organization, Bet Dagan, Israel
Bogol, V., Volcani Center, Institute of Plant Sciences, Agricultural Research Organization, Bet Dagan, Israel
Daron, O., Department of Life Sciences, Ben-Gurion University, Beer-Sheva, Israel
Aloni, R., Department of Plant Sciences, Tel Aviv University, Tel Aviv, Israel
Fernie, A.R., Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany
Granot, D., Volcani Center, Institute of Plant Sciences, Agricultural Research Organization, Bet Dagan, Israel
Arabidopsis fructokinases are important for seed oil accumulation and vascular development
Sucrose (a disaccharide made of glucose and fructose) is the primary carbon source transported to sink organs in many plants. Since fructose accounts for half of the hexoses used for metabolism in sink tissues, plant fructokinases (FRKs), the main fructose-phosphorylating enzymes, are likely to play a central role in plant development. However, to date, their specific functions have been the subject of only limited study. The Arabidopsis genome contains seven genes encoding six cytosolic FRKs and a single plastidic FRK. T-DNA knockout mutants for five of the seven FRKs were identified and used in this study. Single knockouts of the FRK mutants did not exhibit any unusual phenotype. Double-mutants of AtFRK6 (plastidic) and AtFRK7 showed normal growth in soil, but yielded dark, distorted seeds. The seed distortion could be complemented by expression of the well-characterized tomato SlFRK1, confirming that a lack of FRK activity was the primary cause of the seed phenotype. Seeds of the double-mutant germinated, but failed to establish on 1/2 MS plates. Seed establishment was made possible by the addition of glucose or sucrose, indicating reduced seed storage reserves. Metabolic profiling of the double-mutant seeds revealed decreased TCA cycle metabolites and reduced fatty acid metabolism. Examination of the mutant embryo cells revealed smaller oil bodies, the primary storage reserve in Arabidopsis seeds. Quadruple and penta FRK mutants showed growth inhibition and leaf wilting. Anatomical analysis revealed smaller trachea elements and smaller xylem area, accompanied by necrosis around the cambium and the phloem. These results demonstrate overlapping and complementary roles of the plastidic AtFRK6 and the cytosolic AtFRK7 in seed storage accumulation, and the importance of AtFRKs for vascular development. © 2017 Stein, Avin-Wittenberg, Krahnert, Zemach, Bogol, Daron, Aloni, Fernie and Granot.
Scientific Publication
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