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Expression of Arabidopsis hexokinase in citrus guard cells controls stomatal aperture and reduces transpiration
Year:
2015
Source of publication :
Frontiers in Plant Science
Authors :
Alchanatis, Victor
;
.
Carmi, Nir
;
.
Fidel, Lena
;
.
Granot, David
;
.
Kelly, Gilor
;
.
Levi, Aharon
;
.
Lugassi, Nitsan
;
.
Raveh, Eran
;
.
Yaniv, Yossi
;
.
Volume :
6
Co-Authors:




Attia, Z., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture The Robert H. Smith Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Moshelion, M., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture The Robert H. Smith Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel

 

Facilitators :
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Total pages:
1
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Abstract:
Hexokinase (HXK) is a sugar-phosphorylating enzyme involved in sugar-sensing. It has recently been shown that HXK in guard cells mediates stomatal closure and coordinates photosynthesis with transpiration in the annual species tomato and Arabidopsis. To examine the role of HXK in the control of the stomatal movement of perennial plants, we generated citrus plants that express Arabidopsis HXK1 (AtHXK1) under KST1, a guard cell-specific promoter. The expression of KST1 in the guard cells of citrus plants has been verified using GFP as a reporter gene. The expression of AtHXK1 in the guard cells of citrus reduced stomatal conductance and transpiration with no negative effect on the rate of photosynthesis, leading to increased water-use efficiency. The effects of light intensity and humidity on stomatal behavior were examined in rooted leaves of the citrus plants. The optimal intensity of photosynthetically active radiation and lower humidity enhanced stomatal closure of AtHXK1 -expressing leaves, supporting the role of sugar in the regulation of citrus stomata. These results suggest that HXK coordinates photosynthesis and transpiration and stimulates stomatal closure not only in annual species, but also in perennial species. © 2015 Lugassi, Kelly, Fidel, Yaniv, Attia, Levi, Alchanatis, Moshelion, Raveh, Carmi and Granot.
Note:
Related Files :
Citrus rooted leaves
hexokinase
humidity
Light intensity
stomata
sugar
transpiration
Water-use efficiency
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More details
DOI :
10.3389/fpls.2015.01114
Article number:
1114
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28318
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:38
Scientific Publication
Expression of Arabidopsis hexokinase in citrus guard cells controls stomatal aperture and reduces transpiration
6




Attia, Z., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture The Robert H. Smith Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Moshelion, M., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture The Robert H. Smith Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel

 

Expression of Arabidopsis hexokinase in citrus guard cells controls stomatal aperture and reduces transpiration
Hexokinase (HXK) is a sugar-phosphorylating enzyme involved in sugar-sensing. It has recently been shown that HXK in guard cells mediates stomatal closure and coordinates photosynthesis with transpiration in the annual species tomato and Arabidopsis. To examine the role of HXK in the control of the stomatal movement of perennial plants, we generated citrus plants that express Arabidopsis HXK1 (AtHXK1) under KST1, a guard cell-specific promoter. The expression of KST1 in the guard cells of citrus plants has been verified using GFP as a reporter gene. The expression of AtHXK1 in the guard cells of citrus reduced stomatal conductance and transpiration with no negative effect on the rate of photosynthesis, leading to increased water-use efficiency. The effects of light intensity and humidity on stomatal behavior were examined in rooted leaves of the citrus plants. The optimal intensity of photosynthetically active radiation and lower humidity enhanced stomatal closure of AtHXK1 -expressing leaves, supporting the role of sugar in the regulation of citrus stomata. These results suggest that HXK coordinates photosynthesis and transpiration and stimulates stomatal closure not only in annual species, but also in perennial species. © 2015 Lugassi, Kelly, Fidel, Yaniv, Attia, Levi, Alchanatis, Moshelion, Raveh, Carmi and Granot.
Scientific Publication
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