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Abscission of flowers and floral organs is closely associated with alkalization of the cytosol in abscission zone cells
Year:
2015
Source of publication :
Journal of Experimental Botany
Authors :
Belausov, Eduard
;
.
Kochanek, Bettina
;
.
Meir, Shimon
;
.
Philosoph-Hadas, Sonia
;
.
Sundaresan, Srivignesh
;
.
Volume :
66
Co-Authors:
Sundaresan, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel, Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Philosoph-Hadas, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Riov, J., Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Belausov, E., Department of Ornamental Horticulture, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Kochanek, B., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Tucker, M.L., Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD, United States
Meir, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Facilitators :
From page:
1355
To page:
1368
(
Total pages:
14
)
Abstract:
In vivo changes in the cytosolic pH of abscission zone (AZ) cells were visualized using confocal microscopic detection of the fluorescent pH-sensitive and intracellularly trapped dye, 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF), driven by its acetoxymethyl ester. A specific and gradual increase in the cytosolic pH of AZ cells was observed during natural abscission of flower organs in Arabidopsis thaliana and wild rocket (Diplotaxis tenuifolia), and during flower pedicel abscission induced by flower removal in tomato (Solanum lycopersicum Mill). The alkalization pattern in the first two species paralleled the acceleration or inhibition of flower organ abscission induced by ethylene or its inhibitor 1-methylcyclopropene (1-MCP), respectively. Similarly, 1-MCP pre-treatment of tomato inflorescence explants abolished the pH increase in AZ cells and pedicel abscission induced by flower removal. Examination of the pH changes in the AZ cells of Arabidopsis mutants defective in both ethylene-induced (ctr1, ein2, eto4) and ethylene-independent (ida, nev7, dab5) abscission pathways confirmed these results. The data indicate that the pH changes in the AZ cells are part of both the ethylene-sensitive and -insensitive abscission pathways, and occur concomitantly with the execution of organ abscission. pH can affect enzymatic activities and/or act as a signal for gene expression. Changes in pH during abscission could occur via regulation of transporters in AZ cells, which might affect cytosolic pH. Indeed, four genes associated with pH regulation, vacuolar H+-ATPase, putative high-affinity nitrate transporter, and two GTP-binding proteins, were specifically up-regulated in tomato flower AZ following abscission induction, and 1-MCP reduced or abolished the increased expression. © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.
Note:
Related Files :
Abscission zone
arabidopsis
Arabidopsis mutants
Arabidopsis thaliana
ethylene
Flower organs
pH regulation
Show More
Related Content
More details
DOI :
10.1093/jxb/eru483
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28341
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:38
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Scientific Publication
Abscission of flowers and floral organs is closely associated with alkalization of the cytosol in abscission zone cells
66
Sundaresan, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel, Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Philosoph-Hadas, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Riov, J., Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Belausov, E., Department of Ornamental Horticulture, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Kochanek, B., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Tucker, M.L., Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD, United States
Meir, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Abscission of flowers and floral organs is closely associated with alkalization of the cytosol in abscission zone cells
In vivo changes in the cytosolic pH of abscission zone (AZ) cells were visualized using confocal microscopic detection of the fluorescent pH-sensitive and intracellularly trapped dye, 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF), driven by its acetoxymethyl ester. A specific and gradual increase in the cytosolic pH of AZ cells was observed during natural abscission of flower organs in Arabidopsis thaliana and wild rocket (Diplotaxis tenuifolia), and during flower pedicel abscission induced by flower removal in tomato (Solanum lycopersicum Mill). The alkalization pattern in the first two species paralleled the acceleration or inhibition of flower organ abscission induced by ethylene or its inhibitor 1-methylcyclopropene (1-MCP), respectively. Similarly, 1-MCP pre-treatment of tomato inflorescence explants abolished the pH increase in AZ cells and pedicel abscission induced by flower removal. Examination of the pH changes in the AZ cells of Arabidopsis mutants defective in both ethylene-induced (ctr1, ein2, eto4) and ethylene-independent (ida, nev7, dab5) abscission pathways confirmed these results. The data indicate that the pH changes in the AZ cells are part of both the ethylene-sensitive and -insensitive abscission pathways, and occur concomitantly with the execution of organ abscission. pH can affect enzymatic activities and/or act as a signal for gene expression. Changes in pH during abscission could occur via regulation of transporters in AZ cells, which might affect cytosolic pH. Indeed, four genes associated with pH regulation, vacuolar H+-ATPase, putative high-affinity nitrate transporter, and two GTP-binding proteins, were specifically up-regulated in tomato flower AZ following abscission induction, and 1-MCP reduced or abolished the increased expression. © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.
Scientific Publication
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