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Determination of pathogen-related enzyme action by mass spectrometry analysis of pectin breakdown products of plant cell walls
Year:
2005
Source of publication :
Analytical Biochemistry
Authors :
Lurie, Susan
;
.
Volume :
338
Co-Authors:
An, H.J., Department of Chemistry, University of California, Davis, CA 95616, United States
Lurie, S., Department of Postharvest Science, Volcani Center, Bet-Dagan, Israel
Greve, L.C., Department of Pomology, University of California, Davis, CA 95616, United States
Rosenquist, D., Department of Chemistry, University of California, Davis, CA 95616, United States
Kirmiz, C., Department of Chemistry, University of California, Davis, CA 95616, United States
Labavitch, J.M., Department of Pomology, University of California, Davis, CA 95616, United States
Lebrilla, C.B., Department of Chemistry, University of California, Davis, CA 95616, United States
Facilitators :
From page:
71
To page:
82
(
Total pages:
12
)
Abstract:
An analytical approach using matrix-assisted laser desorption/ionization mass spectrometry for the structural characterization and assessment of the degree of polymerization of cell wall pectin-derived oligosaccharides (PDOs) in three regions of Botrytis cinerea-infected tomato fruit tissue is described. The PDOs were isolated from lesion centers (extensively macerated tissue), the area just beyond visible lesion margins, and healthy and intact tissue of an inoculated fruit, sampled at a distance from developing lesions. PDO mixtures were directly analyzed by mass spectrometry without chromatographic separation, after minimum cleanup by membrane drop dialysis. The structures identified implied the action of three different pathogen pectin-modifying enzymes. Modifications such as methyl esterification were identified by determination of exact PDO molecular masses and tandem mass spectrometry via collision-induced dissociation. We have identified four PDO series that were generated through the breakdown of homogalacturonan pectins. The decayed and lesion edge areas had fewer and less diverse PDOs than healthy tissues, possibly due to metabolic by-products of the pathogen. This analytical technique provides a simple and rapid method to characterize the pectin-derived oligosaccharides produced by in vivo digestion during pathogen infection. © 2004 Elsevier Inc. All rights reserved.
Note:
Related Files :
Botrytis
chromatography
Chromatography, High Pressure Liquid
enzyme
Hexuronic Acids
Inoculation
Plant Cell
sensitivity and specificity
Show More
Related Content
More details
DOI :
10.1016/j.ab.2004.11.004
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28410
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:39
Scientific Publication
Determination of pathogen-related enzyme action by mass spectrometry analysis of pectin breakdown products of plant cell walls
338
An, H.J., Department of Chemistry, University of California, Davis, CA 95616, United States
Lurie, S., Department of Postharvest Science, Volcani Center, Bet-Dagan, Israel
Greve, L.C., Department of Pomology, University of California, Davis, CA 95616, United States
Rosenquist, D., Department of Chemistry, University of California, Davis, CA 95616, United States
Kirmiz, C., Department of Chemistry, University of California, Davis, CA 95616, United States
Labavitch, J.M., Department of Pomology, University of California, Davis, CA 95616, United States
Lebrilla, C.B., Department of Chemistry, University of California, Davis, CA 95616, United States
Determination of pathogen-related enzyme action by mass spectrometry analysis of pectin breakdown products of plant cell walls
An analytical approach using matrix-assisted laser desorption/ionization mass spectrometry for the structural characterization and assessment of the degree of polymerization of cell wall pectin-derived oligosaccharides (PDOs) in three regions of Botrytis cinerea-infected tomato fruit tissue is described. The PDOs were isolated from lesion centers (extensively macerated tissue), the area just beyond visible lesion margins, and healthy and intact tissue of an inoculated fruit, sampled at a distance from developing lesions. PDO mixtures were directly analyzed by mass spectrometry without chromatographic separation, after minimum cleanup by membrane drop dialysis. The structures identified implied the action of three different pathogen pectin-modifying enzymes. Modifications such as methyl esterification were identified by determination of exact PDO molecular masses and tandem mass spectrometry via collision-induced dissociation. We have identified four PDO series that were generated through the breakdown of homogalacturonan pectins. The decayed and lesion edge areas had fewer and less diverse PDOs than healthy tissues, possibly due to metabolic by-products of the pathogen. This analytical technique provides a simple and rapid method to characterize the pectin-derived oligosaccharides produced by in vivo digestion during pathogen infection. © 2004 Elsevier Inc. All rights reserved.
Scientific Publication
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