Co-Authors:
An, H.J., Department of Chemistry, University of California, Davis, CA 95616, United States
Lurie, S., Department of Postharvest Science, Volcani Center, Bet-Dagan, Israel
Greve, L.C., Department of Pomology, University of California, Davis, CA 95616, United States
Rosenquist, D., Department of Chemistry, University of California, Davis, CA 95616, United States
Kirmiz, C., Department of Chemistry, University of California, Davis, CA 95616, United States
Labavitch, J.M., Department of Pomology, University of California, Davis, CA 95616, United States
Lebrilla, C.B., Department of Chemistry, University of California, Davis, CA 95616, United States
Abstract:
An analytical approach using matrix-assisted laser desorption/ionization mass spectrometry for the structural characterization and assessment of the degree of polymerization of cell wall pectin-derived oligosaccharides (PDOs) in three regions of Botrytis cinerea-infected tomato fruit tissue is described. The PDOs were isolated from lesion centers (extensively macerated tissue), the area just beyond visible lesion margins, and healthy and intact tissue of an inoculated fruit, sampled at a distance from developing lesions. PDO mixtures were directly analyzed by mass spectrometry without chromatographic separation, after minimum cleanup by membrane drop dialysis. The structures identified implied the action of three different pathogen pectin-modifying enzymes. Modifications such as methyl esterification were identified by determination of exact PDO molecular masses and tandem mass spectrometry via collision-induced dissociation. We have identified four PDO series that were generated through the breakdown of homogalacturonan pectins. The decayed and lesion edge areas had fewer and less diverse PDOs than healthy tissues, possibly due to metabolic by-products of the pathogen. This analytical technique provides a simple and rapid method to characterize the pectin-derived oligosaccharides produced by in vivo digestion during pathogen infection. © 2004 Elsevier Inc. All rights reserved.
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