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A new pathotype of Pepper mild mottle virus (PMMoV) overcomes the L 4 resistance genotype of pepper cultivars
Year:
2008
Source of publication :
Plant Disease
Authors :
Antignus, Yeheskel
;
.
Lachman, Oded
;
.
Maslenin, Ludmila
;
.
Pearlsman, Malenia
;
.
Rosner, Arie
;
.
Volume :
92
Co-Authors:
Antignus, Y., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Lachman, O., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Pearlsman, M., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Rosner, A., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
1033
To page:
1037
(
Total pages:
5
)
Abstract:
The biological, serological, and molecular characteristics of a newly isolated L4 resistance-breaking isolate of Pepper mild mottle virus (PMMoV) were studied. The new pathotype of PMMoV is closely related to the Israeli pathotypes P1,2 and P1,2,3 of the virus; however, the mosaic symptoms caused by this new pathotype on pepper plants with an L 4 genotype were more severe than the mild mosaic symptoms caused by other common pathotypes of the virus in susceptible plants. The predicted amino acid sequence of the putative coat protein (CP) of the newly described pathotype has two amino acid mismatches when compared with the CP of pathotype P 1,2, leucine to glutamine at position 47, and alanine to glycine at position 87. The CP of the new pathotype has one amino acid mismatch when compared with P1,2,3, having alanine instead of glycine at position 87. Based on its biological characteristics, the new pathotype was designated P1,2,3,4 of PMMoV-Is. A method is described for the differentiation among the three PMMoV pathotypes using restriction cleavage analysis of reverse-transcription polymerase chain reaction products made from virus-infected plants. An additional unique MnlI site in the CP gene of the newly isolated P1,2,3,4 allows its distinction from the other two isolates, while BglI cleaved only products of the P1,2 pathotype. © 2008 The American Phytopathological Society.
Note:
Related Files :
Molecular characteristics
Pathotype
Pepper mild mottle virus
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Related Content
More details
DOI :
10.1094/PDIS-92-7-1033
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28414
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:39
You may also be interested in
Scientific Publication
A new pathotype of Pepper mild mottle virus (PMMoV) overcomes the L 4 resistance genotype of pepper cultivars
92
Antignus, Y., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Lachman, O., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Pearlsman, M., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Rosner, A., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
A new pathotype of Pepper mild mottle virus (PMMoV) overcomes the L 4 resistance genotype of pepper cultivars
The biological, serological, and molecular characteristics of a newly isolated L4 resistance-breaking isolate of Pepper mild mottle virus (PMMoV) were studied. The new pathotype of PMMoV is closely related to the Israeli pathotypes P1,2 and P1,2,3 of the virus; however, the mosaic symptoms caused by this new pathotype on pepper plants with an L 4 genotype were more severe than the mild mosaic symptoms caused by other common pathotypes of the virus in susceptible plants. The predicted amino acid sequence of the putative coat protein (CP) of the newly described pathotype has two amino acid mismatches when compared with the CP of pathotype P 1,2, leucine to glutamine at position 47, and alanine to glycine at position 87. The CP of the new pathotype has one amino acid mismatch when compared with P1,2,3, having alanine instead of glycine at position 87. Based on its biological characteristics, the new pathotype was designated P1,2,3,4 of PMMoV-Is. A method is described for the differentiation among the three PMMoV pathotypes using restriction cleavage analysis of reverse-transcription polymerase chain reaction products made from virus-infected plants. An additional unique MnlI site in the CP gene of the newly isolated P1,2,3,4 allows its distinction from the other two isolates, while BglI cleaved only products of the P1,2 pathotype. © 2008 The American Phytopathological Society.
Scientific Publication
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