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Biolistic DNA delivery to leaf tissue of plants with the non-vacuum gene gun (HandyGun)
Year:
2013
Source of publication :
Methods in Molecular Biology
Authors :
Gaba, Victor
;
.
Volume :
940
Co-Authors:
Vuorinen, A.L., Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland
Nieminen, A., Instrument Center, University of Helsinki, Helsinki, Finland
Gaba, V., Department of Plant Pathology and Weed Science, Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Sikorskaite, S., Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland
Valkonen, J.P.T., Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland
Facilitators :
From page:
45
To page:
51
(
Total pages:
7
)
Abstract:
Non-vacuum gene guns such as HandyGun are flexible tools for bombardment of targets of varying size. Construction of HandyGun is simpler and cheaper than vacuum gene guns and will be described here. The conditions for maximal transient transformation efficiency of plant cells with plasmid DNA using HandyGun will be provided. © 2013 Springer Science+Business Media, LLC.
Note:
Related Files :
article
Biolistics
biolistic transformation
cytology
DNA
Genetics
HandyGun
Infectious virus clone
Plant Leaves
Show More
Related Content
More details
DOI :
10.1007/978-1-62703-110-3_4
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28429
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:39
Scientific Publication
Biolistic DNA delivery to leaf tissue of plants with the non-vacuum gene gun (HandyGun)
940
Vuorinen, A.L., Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland
Nieminen, A., Instrument Center, University of Helsinki, Helsinki, Finland
Gaba, V., Department of Plant Pathology and Weed Science, Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Sikorskaite, S., Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland
Valkonen, J.P.T., Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland
Biolistic DNA delivery to leaf tissue of plants with the non-vacuum gene gun (HandyGun)
Non-vacuum gene guns such as HandyGun are flexible tools for bombardment of targets of varying size. Construction of HandyGun is simpler and cheaper than vacuum gene guns and will be described here. The conditions for maximal transient transformation efficiency of plant cells with plasmid DNA using HandyGun will be provided. © 2013 Springer Science+Business Media, LLC.
Scientific Publication
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